Anti-proliferative effect of camellianin A in Adinandra nitida leaves and its apoptotic induction in human Hep G2 and MCF-7 cells.

Abstract

Leaves of Adinandra nitida constitute a kind of flavonoid-rich plant food. In this study, camellianin A, the main flavonoid in the leaves of Adinandra nitida,was prepared and identified by high performance liquid chromatography-photodiode array detector-electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). In the anticancer assay, it was found camellianin A could inhibit the proliferation of the human hepatocellular liver carcinoma Hep G2 and human breast adenocarcinoma MCF-7 cell lines in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treated by camellianin A, phosphatidylserine of Hep G2 and MCF-7 cells could translocate significantly to the surface of the membrane. The increase of an early apoptotic population of Hep G2 and MCF-7 cells was observed. It was concluded that camellianin A not only affected the progress of the cell cycle, but also induced cells to enter into apoptosis.

• Authors: Han Gao, Benguo Liu, Feng Liu, Yongsheng Chen
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 15 Issue 6 Pg. 3878-86 (May 28 2010) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 20657414 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Flavonoids
• camellianin A

Topics

• Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
• Apoptosis (drug effects)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Flavonoids (chemistry, pharmacology)
• Hep G2 Cells
• Humans
• Molecular Structure
• Plant Leaves (chemistry)
• Plants, Medicinal (chemistry)