Immunogenicity of the Plasmodium falciparum Pf332-DBL domain in combination with different adjuvants.

Abstract	The Plasmodium falciparum antigen 332 (Pf332) is a conserved blood-stage antigen, which has been suggested to play a role in parasite invasion. In the present study, we have investigated the immunogenicity of the Duffy-binding like (DBL)-domain of the Pf332 molecule in combination with different adjuvants in four animal species. Three of the adjuvants are applicable for human use (Montanide ISA 720, alum and levamisole), whilst Freund's adjuvant served as a positive control adjuvant. Montanide ISA 720 was able to generate a significant and Th2-biased IgG response in BALB/c and C57BL/6 mice. Alum was a strong inducer of a Th2-type immune response only in BALB/c mice, whereas it was a poor adjuvant together with Pf332-DBL in C57BL/6 mice, rabbits and rats. Levamisole did not show any obvious adjuvant effect in any of the immunized animals. Thus in the case with Pf332-DBL, Montanide ISA 720 may be an adjuvant to further explore in the development of a vaccine against malaria.
Authors	Cheng Du, Sandra Nilsson, Huijun Lu, Jigang Yin, Ning Jiang, Mats Wahlgren, Qijun Chen
Journal	Vaccine (Vaccine) Vol. 28 Issue 31 Pg. 4977-83 (Jul 12 2010) ISSN: 1873-2518 [Electronic] Netherlands
PMID	20653104 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Adjuvants, Immunologic Alum Compounds Antibodies, Protozoan Immunoglobulin G Malaria Vaccines Oleic Acids Protozoan Proteins antigen Pf332, Plasmodium mannide monooleate Levamisole aluminum sulfate Mannitol Freund's Adjuvant
Topics	Adjuvants, Immunologic (pharmacology) Alum Compounds (pharmacology) Animals Antibodies, Protozoan (blood) Antibody Formation Freund's Adjuvant (pharmacology) Immunoglobulin G (blood) Levamisole (pharmacology) Malaria Vaccines (immunology) Mannitol (analogs & derivatives, pharmacology) Mice Mice, Inbred BALB C Mice, Inbred C57BL Oleic Acids (pharmacology) Plasmodium falciparum (immunology) Protozoan Proteins (immunology) Rabbits Rats Rats, Sprague-Dawley

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