Biologic markers for odontogenic periradicular periodontitis.

Abstract

INTRODUCTION:

The diagnosis and assessment of apical periodontitis by traditional periapical radiographs can be challenging and might yield false-negative results. The aim of this study was to determine whether interleukin-1beta (IL-1beta) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) can be used as biological markers for apical periodontitis.

METHODS:

Forty healthy patients with teeth diagnosed with apical periodontitis of pulpal origin were included in the study. GCF samples were obtained from the diseased tooth and from a healthy contralateral control tooth. Total protein concentration in each sample was determined by using the Bio-Rad protein assay. Enzyme-linked immunosorbent assay was used to analyze the concentration of IL-1beta and DSP in the samples.

RESULTS:

Protein content of the GCF was statistically significantly higher in the disease group compared with the control group. The levels of IL-1beta and DSP were not statistically different between disease and control groups.

CONCLUSIONS:

Although this study was unable to demonstrate a significantly higher level of IL-1beta or DSP in the GCF of teeth with apical periodontitis, the observed presence of a significantly higher level of total protein in the GCF of diseased teeth suggests the possible role of total protein level as a marker for periapical disease.

• Authors: Bruna Burgener, Angelique R Ford, Hongsa Situ, Mohamed I Fayad, Jian Jun Hao, Christopher S Wenckus, Bradford R Johnson, Ellen A BeGole, Anne George
• Journal: Journal of endodontics (J Endod) Vol. 36 Issue 8 Pg. 1307-10 (Aug 2010) ISSN: 1878-3554 [Electronic] United States
• PMID: 20647085 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• Extracellular Matrix Proteins
• Interleukin-1beta
• Phosphoproteins
• Proteins
• Sialoglycoproteins
• dentin sialophosphoprotein

Topics

• Biomarkers (analysis)
• Extracellular Matrix Proteins (analysis)
• Female
• Gingival Crevicular Fluid (chemistry)
• Humans
• Interleukin-1beta (analysis)
• Male
• Periapical Periodontitis (diagnosis, metabolism)
• Phosphoproteins (analysis)
• Proteins (analysis)
• Sialoglycoproteins (analysis)