Manzamine A as a novel inhibitor of herpes simplex virus type-1 replication in cultured corneal cells.

Abstract

This study investigated the putative inhibitory effect of manzamine A on HSV-1 infection. Our results indicated that manzamine A effectively inhibited viral replication and infection in the cell line SIRC, a corneal cell line, at 1 µM. The existing anti-HSV-1 drug acyclovir was analyzed and showed a comparable activity at 50 µM. Plaque assays demonstrated that manzamine A reduced the release of infectious virus by 10 (11)-fold. RTPCR assays indicated that HSV-1 virion host shutoff (vhs) activity and ICP0 transcription were decreased by manzamine A treatment. These results bode well for the development of manzamines as potential leads to reduce viral infection in corneal cells and to prevent HSV-1-induced eye infections such as keratitis.

• Authors: Jayavardhana R Palem, Gautam R Bedadala, Khalid A El Sayed, Shao-chung V Hsia
• Journal: Planta medica (Planta Med) Vol. 77 Issue 1 Pg. 46-51 (Jan 2011) ISSN: 1439-0221 [Electronic] Germany
• PMID: 20645244 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Antiviral Agents
• Carbazoles
• manzamine A

Topics

• Animals
• Antiviral Agents (chemistry, isolation & purification, pharmacology)
• Carbazoles (chemistry, isolation & purification, pharmacology)
• Cell Line
• Cornea (virology)
• Herpesvirus 1, Human (drug effects)
• Porifera (chemistry)
• Rabbits
• Virus Replication (drug effects)