Abstract |
We hypothesized that maternal creatine supplementation from mid-pregnancy would protect the diaphragm of the newborn spiny mouse from the effects of intrapartum hypoxia. Pregnant mice were fed a control or 5% creatine-supplemented diet from mid-gestation. On the day before term, intrapartum hypoxia was induced by isolating the pregnant uterus in a saline bath for 7.5-8 min before releasing and resuscitating the fetuses. Surviving pups were placed with a cross-foster dam, and diaphragm tissue was collected at 24 h postnatal age. Hypoxia caused a significant decrease in the cross-sectional area (∼19%) and contractile function (26.6% decrease in maximum Ca2=-activated force) of diaphragm fibers. The mRNA levels of the muscle mass-regulating genes MuRF1 and myostatin were significantly increased (2-fold). Maternal creatine significantly attenuated hypoxia-induced fiber atrophy, contractile dysfunction, and changes in mRNA levels. This study demonstrates that creatine loading before birth significantly protects the diaphragm from hypoxia-induced damage at birth.
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Authors | David J Cannata, Zoe Ireland, Hayley Dickinson, Rod J Snow, Aaron P Russell, Jan M West, David W Walker |
Journal | Pediatric research
(Pediatr Res)
Vol. 68
Issue 5
Pg. 393-8
(Nov 2010)
ISSN: 1530-0447 [Electronic] United States |
PMID | 20639795
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Animals, Newborn
- Creatine
(administration & dosage, pharmacology)
- Diaphragm
(cytology, drug effects, pathology)
- Diet
- Dietary Supplements
- Female
- Fetal Hypoxia
(pathology, physiopathology)
- Fetus
(anatomy & histology, drug effects, pathology)
- Gestational Age
- Mice
- Muscle Contraction
(drug effects, physiology)
- Muscle, Skeletal
(cytology, drug effects, metabolism, pathology)
- Pregnancy
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