Abstract |
Arthroplasty is a very successful medical procedure. Failures depend on aseptic loosening caused by periprosthetic osteolysis, where T cells have a contradictory role. We analyzed osteoclastogenesis in peripheral blood mononuclear cell (PBMC) cultures of periprosthetic osteolysis patients and the phenotype of T cells localized in periprosthetic tissues. We enrolled 45 subjects with periprosthetic osteolysis (15), stable prosthesis (15) and healthy controls (15). We performed PBMC cultures to study osteoclastogenesis. Osteoclasts and T cell phenotype were examined by immunohistochemistry, immunofluorescence and flow citometry. Periprosthetic osteolysis patients showed spontaneous osteoclastogenesis, which was inhibited by RANK-Fc and T cell depletion. In periprosthetic osteolysis patients' PBMC cultures, CD4 and CD8 T cells increased and CD8 T cells did not express CD25. In periprosthetic tissues T cells were close to osteoclasts, suggesting their interaction. Local CD8 T cells showed a regulatory phenotype, expressing CD25 and FoxP3, while CD4 T cells did not express activation markers. Our data suggest that, in an early stage of periprosthetic osteolysis, T cells may promote osteoclastogenesis, whereas subsequently osteoclasts activate FoxP3/CD8 T cells, which inhibit CD4 effector T cells. This mechanism may explain the previous finding of non-active T cells in periprosthetic tissues.
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Authors | Ilaria Roato, Davide Caldo, Lucia D'Amico, Patrizia D'Amelio, Laura Godio, Salvatore Patanè, Franco Astore, Guido Grappiolo, Maurizio Boggio, Roberto Scagnelli, Luigi Molfetta, Riccardo Ferracini |
Journal | Biomaterials
(Biomaterials)
Vol. 31
Issue 29
Pg. 7519-25
(Oct 2010)
ISSN: 1878-5905 [Electronic] Netherlands |
PMID | 20638717
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Interleukin-2 Receptor alpha Subunit
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Topics |
- Arthroplasty, Replacement, Hip
(adverse effects)
- CD4-Positive T-Lymphocytes
(metabolism)
- CD8-Positive T-Lymphocytes
(metabolism)
- Cells, Cultured
- Flow Cytometry
- Fluorescent Antibody Technique
- Humans
- Immunohistochemistry
- Interleukin-2 Receptor alpha Subunit
(metabolism)
- Leukocytes, Mononuclear
(metabolism, pathology)
- Osteoclasts
(metabolism, pathology)
- Osteolysis
(etiology, immunology, metabolism, pathology)
- T-Lymphocytes
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