Abstract | BACKGROUND AND AIMS: METHODS: UC was induced in mice by oral administration of synthetic DSS (molecular weight 5000) for 7 days. Mice were divided into normal group, colitis control group, low-dose Res-treated group (RLD-treated group), and high-dose Res-treated group (RHD-treated group). Inhibitory effects of concomitant treatment with Res were assessed daily using a Disease Activity Index (DAI) and severity of histological changes. MDA, MPO, SOD and GSH-PX activity of colonic tissue were determined in colon samples by chemical colorimetry. TNF-alpha, IL-8, IFN-gamma, p22( phox) and gp91( phox) expression levels were detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), ELISA, and Western blot analysis. RESULT: Administration of Res significantly inhibited the severity of UC compared to the colitis control group. Colonic tissue MDA and MPO activities decreased significantly in Res-treated groups compared to colitis control groups. Furthermore, colonic tissue SOD and GSH-Px activities increased significantly in Res-treated groups compared to colitis control groups. The expression levels of TNF-alpha, IL-8, IFN-gamma, p22( phox), and gp91( phox) also decreased significantly in the Res-treated group compared to the colitis control group. CONCLUSIONS:
Oral administration of Res exerts marked inhibitory effects on UC in mice. Resveratrol may play an important role in preventing DSS-induced oxidative damage.
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Authors | Jun Yao, Jian-Yao Wang, Lei Liu, Ying-Xue Li, An-Ying Xun, Wei-Sen Zeng, Chun-Hong Jia, Xiao-Xia Wei, Ju-Ling Feng, Li Zhao, Li-Sheng Wang |
Journal | Archives of medical research
(Arch Med Res)
Vol. 41
Issue 4
Pg. 288-94
(May 2010)
ISSN: 1873-5487 [Electronic] United States |
PMID | 20637373
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 IMSS. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Antioxidants
- DNA Primers
- Reactive Oxygen Species
- Stilbenes
- Malondialdehyde
- Dextran Sulfate
- Peroxidase
- Glutathione Peroxidase
- Resveratrol
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Base Sequence
- Blotting, Western
- Colitis, Ulcerative
(chemically induced, enzymology, metabolism)
- Colon
(enzymology, metabolism)
- DNA Primers
- Dextran Sulfate
(toxicity)
- Enzyme-Linked Immunosorbent Assay
- Glutathione Peroxidase
(metabolism)
- Male
- Malondialdehyde
(metabolism)
- Mice
- Mice, Inbred BALB C
- Peroxidase
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Resveratrol
- Reverse Transcriptase Polymerase Chain Reaction
- Stilbenes
(pharmacology)
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