The orally combined neuroprotective effects of sodium ferulate and borneol against transient global ischaemia in C57 BL/6J mice.

Abstract

OBJECTIVES:

This study aimed to investigate the possible modification of the neuroprotective effect of sodium ferulate, when orally co-administered with borneol, in transient global cerebral ischaemia-induced functional, histological and cellular alterations in mice.

METHODS:

The bilateral common carotid artery occlusion was conducted in C57 BL/6J mice for 25 min. The mice were then subjected to a water maze test over an extended recovery period, followed by an assessment of neuronal loss in the CA1 region of the hippocampus (haematoxylin and eosin staining). The blood-brain barrier permeability (Evans blue tracing), brain oedema and oxidative stress were assayed and histological sections were also immunostained for gliofibrillar acid protein (GFAP) expression.

KEY FINDINGS:

The ischaemia reperfused mice were associated with long-lasting spatial learning deficits in the absence of other behavioural impairments and with neurodegeneration in the hippocampal CA1 region. However, the histological injuries were significantly attenuated by oral co-administration of sodium ferulate and borneol. Furthermore, combined treatment with sodium ferulate and borneol resulted in a significant reduction in brain oedema, GFAP-positive cells, malonaldialdehyde levels and blood-brain barrier permeability, but an increase in superoxide dismutase activity.

CONCLUSIONS:

Borneol may have benefits for the neuroprotective effect of sodium ferulate against injury induced in the brain by ischaemia/reperfusion.

• Authors: Xiao-Hong Chen, Zhu-Zhen Lin, An-Min Liu, Jian-Tao Ye, Yan Luo, Yu-Yan Luo, Xue-Xuan Mao, Pei-Qing Liu, Rong-Biao Pi
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 62 Issue 7 Pg. 915-23 (07 2010) ISSN: 2042-7158 [Electronic] England
• PMID: 20636880 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Camphanes
• Coumaric Acids
• Glial Fibrillary Acidic Protein
• Nerve Tissue Proteins
• Neuroprotective Agents
• Plant Extracts
• glial fibrillary astrocytic protein, mouse
• Malondialdehyde
• ferulic acid
• Superoxide Dismutase
• isoborneol

Topics

• Animals
• Blood-Brain Barrier (drug effects)
• CA1 Region, Hippocampal (drug effects, metabolism, pathology)
• Camphanes (administration & dosage, pharmacology, therapeutic use)
• Carotid Arteries
• Cerebrovascular Disorders
• Coumaric Acids (administration & dosage, pharmacology, therapeutic use)
• Drug Therapy, Combination
• Edema (drug therapy)
• Glial Fibrillary Acidic Protein
• Ischemic Attack, Transient (drug therapy, metabolism, pathology)
• Learning
• Male
• Malondialdehyde (metabolism)
• Maze Learning
• Mice
• Mice, Inbred C57BL
• Nerve Tissue Proteins (metabolism)
• Neuroprotective Agents (administration & dosage, pharmacology, therapeutic use)
• Permeability
• Plant Extracts (administration & dosage, pharmacology, therapeutic use)
• Reperfusion Injury (drug therapy, metabolism, pathology)
• Superoxide Dismutase (metabolism)