Abstract |
Mitochondria were isolated from a slow-growing (9618A) and two intermediate-to-fast-growing (5123C, 5123tc) Morris hepatomas and host livers. The mitochondrial proteins were solubilized and fractionated on sodium dodecyl sulfate: polyacrylamide slab gels. One Coomassie blue-stained band was absent or reduced in amount in all tumors relative to host livers. In addition, a major mitochondrial enzyme present in normal liver, carbamyl phosphate synthetase, was missing or greatly reduced in the slow-growing, highly differentiated hepatoma 9618A, a tumor that is considered to be similar to normal liver in many biochemical and morphological respects. Incubation of mitochondria with [35S] methionine and a suitable amino acid incorporation system resulted in labeling of specific mitochondrial proteins. Autoradiography of the slab gels disclosed four prominently labeled fractions and a number of minor fractions. Preparations from hepatoma 5123tc demonstrated two labeled bands that were absent or greatly reduced in host liver. Host liver preparations displayed a minor band that was absent or greatly reduced in hepatoma 5123C. However, no single change in labeling pattern was common to all three tumors, suggesting the absence of a causal relationship between carcinogenesis and mutations in mitochondrial DNA.
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Authors | C C Irwin, L I Malkin, H P Morris |
Journal | Cancer research
(Cancer Res)
Vol. 38
Issue 6
Pg. 1584-8
(Jun 1978)
ISSN: 0008-5472 [Print] United States |
PMID | 206352
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Neoplasm
- Neoplasm Proteins
- Proteins
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Topics |
- Animals
- Carcinoma, Hepatocellular
(metabolism)
- DNA, Neoplasm
(metabolism)
- Liver Neoplasms
(metabolism)
- Male
- Mitochondria, Liver
(metabolism)
- Neoplasm Proteins
(biosynthesis, metabolism)
- Neoplasms, Experimental
(metabolism)
- Protein Biosynthesis
- Proteins
(metabolism)
- Rats
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