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Differences in total mitochondrial proteins and proteins synthesized by mitochondria from rat liver and Morris hepatomas 9618A, 5123C, and 5123tc.

Abstract
Mitochondria were isolated from a slow-growing (9618A) and two intermediate-to-fast-growing (5123C, 5123tc) Morris hepatomas and host livers. The mitochondrial proteins were solubilized and fractionated on sodium dodecyl sulfate:polyacrylamide slab gels. One Coomassie blue-stained band was absent or reduced in amount in all tumors relative to host livers. In addition, a major mitochondrial enzyme present in normal liver, carbamyl phosphate synthetase, was missing or greatly reduced in the slow-growing, highly differentiated hepatoma 9618A, a tumor that is considered to be similar to normal liver in many biochemical and morphological respects. Incubation of mitochondria with [35S]methionine and a suitable amino acid incorporation system resulted in labeling of specific mitochondrial proteins. Autoradiography of the slab gels disclosed four prominently labeled fractions and a number of minor fractions. Preparations from hepatoma 5123tc demonstrated two labeled bands that were absent or greatly reduced in host liver. Host liver preparations displayed a minor band that was absent or greatly reduced in hepatoma 5123C. However, no single change in labeling pattern was common to all three tumors, suggesting the absence of a causal relationship between carcinogenesis and mutations in mitochondrial DNA.
AuthorsC C Irwin, L I Malkin, H P Morris
JournalCancer research (Cancer Res) Vol. 38 Issue 6 Pg. 1584-8 (Jun 1978) ISSN: 0008-5472 [Print] United States
PMID206352 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Neoplasm
  • Neoplasm Proteins
  • Proteins
Topics
  • Animals
  • Carcinoma, Hepatocellular (metabolism)
  • DNA, Neoplasm (metabolism)
  • Liver Neoplasms (metabolism)
  • Male
  • Mitochondria, Liver (metabolism)
  • Neoplasm Proteins (biosynthesis, metabolism)
  • Neoplasms, Experimental (metabolism)
  • Protein Biosynthesis
  • Proteins (metabolism)
  • Rats

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