Abstract | INTRODUCTION: Implanted biomaterials could provide surface for bacterial adherence and formation of biofilm, eventually leading to biomaterial-centered infections (BCIs). In this in vitro study, we examined the biofilm-forming capacity of Enterococcus faecalis on gutta-percha (GP) points under different nutrient status and surface conditioning with saliva and serum. METHODS: GP points (n = 420) coated with different root canal sealers ( Sealapex or Roth) were conditioned with saliva or serum for 2-, 4-, and 12-week intervals and subsequently were incubated with E. faecalis for 2 weeks under nutrient-rich and nutrient-deprived environments. The biofilm-forming capacity and the nature of biofilm formed on GP were assessed by using a viable cell assay and scanning electron microscopy (SEM). RESULTS: E. faecalis produced biofilms on GP points (with and without root canal sealers) under both nutrient-rich and nutrient-deprived environments after conditioning with saliva or serum. SEM reflected that the biofilms formed under nutrient-rich conditions were regular, whereas the biofilms formed under nutrient-deprived conditions were irregular and scanty. Under nutrient-deprived conditions, longer conditioning periods in saliva or serum were required for bacterial adherence. CONCLUSIONS: Under the conditions of this study, saliva and serum conditioning of GP points plays an important role in the biofilm formation under tough environmental conditions.
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Authors | Saji George, Bettina Basrani, Anil Kishen |
Journal | Journal of endodontics
(J Endod)
Vol. 36
Issue 7
Pg. 1241-4
(Jul 2010)
ISSN: 1878-3554 [Electronic] United States |
PMID | 20630308
(Publication Type: Journal Article)
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Copyright | Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Culture Media
- Root Canal Filling Materials
- Salicylates
- Zinc Oxide-Eugenol Cement
- Sealapex
- Roth's 801 Sealer
- Gutta-Percha
- Calcium Hydroxide
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Topics |
- Bacterial Adhesion
- Bacteriological Techniques
- Biofilms
- Blood
- Calcium Hydroxide
(chemistry)
- Colony Count, Microbial
- Culture Media
- Enterococcus faecalis
(growth & development, physiology)
- Gram-Positive Bacterial Infections
(microbiology)
- Gutta-Percha
(chemistry)
- Humans
- Materials Testing
- Microscopy, Electron, Scanning
- Root Canal Filling Materials
(chemistry)
- Salicylates
(chemistry)
- Saliva
- Surface Properties
- Time Factors
- Tooth, Nonvital
(microbiology)
- Zinc Oxide-Eugenol Cement
(chemistry)
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