Guinea pigs were sensitized with trinitrophenylated liver macromolecular
protein fraction (TNP-LP1) prepared by using
sodium trinitrobenzenesulfonate of strong immunogenicity as the
hapten and LP1 as the
carrier protein. The administration of trinitrophenylated hepatocytes and
lipopolysaccharide to these TNP-LP1-sensitized guinea pigs through the mesenteric vein 2 weeks later resulted in the induction of
acute hepatic failure accompanied by massive hepatic cell
necrosis in almost all of the guinea pigs. Using this experimental model, the effect of
Gomisin A on the induction of immunological
acute hepatic failure was examined. As a result, the administration of
gomisin A remarkably improved the survival rate and serum
transaminase levels of the immunologically-induced
acute hepatic failure guinea pigs.
Gomisin A also improved the histological changes of the liver in these guinea pigs. These results suggested that
gomisin A is effective for the improvement of immunologically-induced
acute hepatic failure in our experimental model.