Detection of a t(4;14)(p16;q32) in two cases of lymphoma showing both the immunophenotype of chronic lymphocytic leukemia.

Abstract

Reciprocal IGH/14q32 translocations are detectable in 55-70% of patients with plasma cell myeloma; e.g., the adverse t(4;14)(p16;q32) fusing the IGH and FGFR3 genes (immunoglobulin heavy chain/fibroblast growth factor receptor 3). In a minority of patients with B-lineage chronic lymphocytic leukemia (CLL), reciprocal IGH/14q32 translocations have been reported as well. We describe the occurrence of a t(4;14)(p16;q32) in two lymphoma patients showing the immunophenotype of B-CLL, which, to our knowledge, is the first report on such an association. The first patient, a 72-year-old female, showed mature lymphocyte infiltration of the bone marrow and marked splenomegaly. Immunophenotyping revealed aberrant CD5 expression and light-chain lambda restriction on mature B-lymphocytes corresponding to a B-CLL. Interphase fluorescence in situ hybridization (FISH) plus chromosome banding revealed a t(4;14)(p16;q32) in addition to an unbalanced der(16)t(8;16)(q23;p13) and a del(8)(p11). The second patient, a male of 65 years, showed marked peripheral leukocytosis. Immunophenotyping revealed the phenotype of CLL/PLL (chronic lymphocytic leukemia/prolymphocytic leukemia). Again, FISH together with karyotyping revealed a t(4;14)(p16;q32). These two cases with an t(4;14), but the immunophenotype of B-CLL, demonstrate the genetic variability of B-cell lymphomas and the potential of specific metaphase cultivation techniques using oligonucleotides to increase our insights in the genetic pathways of these heterogeneous disorders.

• Authors: Ulrike Bacher, Torsten Haferlach, Susanne Schnittger, Tamara Weiss, Oswald Burkhard, Britta Bechtel, Wolfgang Kern, Claudia Haferlach
• Journal: Cancer genetics and cytogenetics (Cancer Genet Cytogenet) Vol. 200 Issue 2 Pg. 170-4 (Jul 15 2010) ISSN: 1873-4456 [Electronic] United States
• PMID: 20620602 (Publication Type: Case Reports, Journal Article)
• Copyright: Copyright (c) 2010 Elsevier Inc. All rights reserved.

Chemical References

• Immunoglobulin Heavy Chains
• FGFR3 protein, human
• Receptor, Fibroblast Growth Factor, Type 3

Topics

• Aged
• Chromosomes, Human, Pair 14
• Chromosomes, Human, Pair 4
• Female
• Humans
• Immunoglobulin Heavy Chains (genetics)
• Immunophenotyping
• Leukemia, Lymphocytic, Chronic, B-Cell (genetics)
• Lymphoma, B-Cell (genetics)
• Male
• Receptor, Fibroblast Growth Factor, Type 3 (genetics)
• Translocation, Genetic