Antiviral effect of strictinin on influenza virus replication.

Abstract

Strictinin, which is a member of the ellagitanin family of hydrolyzable tannins, prevented replication of human, duck and swine influenza A viruses (IAVs) in vitro at non-toxic concentrations. The addition of strictinin at the same time as IAV inoculation to MDCK cells inhibited viral replication in a dose-dependent manner. Strictinin showed 50% inhibitory concentrations for IAVs from 0.09±0.021 to 0.28±0.037μM (mean±S.E.M.) by the focus-forming assay. Treatment of MDCK cells with strictinin before and after viral inoculation resulted in no significant antiviral activity. Further studies showed that strictinin inhibited IAV-induced hemifusion. However, strictinin exhibited no inhibitory effect against receptor binding, sialidase activity. Strictinin also showed an antiviral effect on influenza B virus and human parainfluenza virus type-1 in vitro. The results indicate that strictinin is a useful antiviral agent.

• Authors: Repon Kumer Saha, Tadanobu Takahashi, Yuuki Kurebayashi, Keijo Fukushima, Akira Minami, Noriaki Kinbara, Masaki Ichitani, Yuko M Sagesaka, Takashi Suzuki
• Journal: Antiviral research (Antiviral Res) Vol. 88 Issue 1 Pg. 10-8 (Oct 2010) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 20615432 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Antiviral Agents
• Phenols
• Tannins
• strictinin
• Neuraminidase

Topics

• Animals
• Antiviral Agents (pharmacology)
• Cell Line
• Dose-Response Relationship, Drug
• Humans
• Influenza A virus (drug effects, physiology)
• Influenza B virus (drug effects, physiology)
• Neuraminidase (antagonists & inhibitors)
• Parainfluenza Virus 1, Human (drug effects, physiology)
• Phenols (pharmacology)
• Tannins (pharmacology)
• Virus Attachment (drug effects)
• Virus Replication (drug effects)