[Effects of laminarin sulphate on the expressions of PTEN and P271kip1 in prostate cancer PC-3 cells].

Abstract

OBJECTIVE:

To study the effect of laminarin sulphate (LAMS) on the expressions of PTEN and P27kip1 in androgen-independent prostate cancer PC-3 cells in vitro, and investigate the mechanism of its anti-tumor action.

METHODS:

The inhibitory effects of different concentrations of LAMS (0, 50, 100, 200 microg/ml) on androgen-independent prostate cancer PC-3 cells were detected by WST-8 assay. The morphology of PC-3 cells was observed under the fluorescence microscope, and the cell cycle and apoptosis were analyzed by flow cytometry. The mRNA and protein levels of PTEN and P27kip1 were measured by RT-PCR and Western blot.

RESULTS:

LAMS inhibited the proliferation of androgen-independent prostate cancer PC-3 cells in a dose- and time-dependent manner, and the cell cycle analysis showed that PC-3 cells were arrested in the S phase after treated with different concentrations of LAMS. The rate of apoptosis was increased and many typical apoptotic morphological features were observed under the fluorescence microscope. The PTEN and P27kip1 expressions at mRNA and protein levels were increased in a dose-dependent manner.

CONCLUSION:

LAMS can inhibit the proliferation, arrest the cell cycle in the S phase and induce apoptosis of prostate cancer PC-3 cells. The significantly increased expressions of PTEN and P27kip1 may be one of the mechanisms for LAMS inhibiting prostate cancer PC-3 cells.

• Authors: Ming-Chang Zou, Fei-Lun Cui, Yu-Qing Sheng, Zhen Qiu
• Journal: Zhonghua nan ke xue = National journal of andrology (Zhonghua Nan Ke Xue) Vol. 16 Issue 6 Pg. 498-503 (Jun 2010) ISSN: 1009-3591 [Print] China
• PMID: 20608352 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Polysaccharides
• RNA, Messenger
• Cyclin-Dependent Kinase Inhibitor p27
• laminarin sulfate
• PTEN Phosphohydrolase
• PTEN protein, human

Topics

• Apoptosis (drug effects)
• Cell Cycle (drug effects)
• Cell Line, Tumor (drug effects)
• Cell Proliferation (drug effects)
• Cyclin-Dependent Kinase Inhibitor p27 (genetics)
• Humans
• Male
• PTEN Phosphohydrolase (genetics)
• Polysaccharides (pharmacology)
• Prostatic Neoplasms (blood, genetics)
• RNA, Messenger (genetics)