Effects of various fragrant ingredients on desmopressin-induced fluid retention in mice.

Abstract

Although fragrances are often used in aromatherapy for the treatment of edema, few studies on their diuretic and/or antiedematous activities have been performed. In this study, the effects of four types of fragrant ingredients (d-limonene, piperitone, alpha-pinene, and cinnamaldehyde) were examined in a mouse model of fluid retention. The mice were loaded with water after treatment with desmopressin (an antidiuretic hormone). In addition, zingerone, a pungent component of ginger which is considered to be effective in the treatment of edema, was examined. Moreover, their effects were compared with those of furosemide, a representative diuretic. Among the five types of fragrant ingredients examined, all except for cinnamaldehyde increased the urine volume in the fluid retention mouse model when administered at a dose of 100 mg/kg. In particular, d-limonene and zingerone significantly increased the urine volume. Thus the effects of these two ingredients were further examined at lower doses of 10 and 30 mg/kg. d-Limonene significantly increased the urine volume in a dose-dependent manner. Zingerone resulted in a significant increase in the urine volume only at a dose of 30 mg/kg. In normal mice, d-limonene did not affect the urine volume at the same doses. In contrast, zingerone significantly increased the urine volume in normal mice at a dose of 30 mg/kg. Furosemide significantly increased the urine volume in both the fluid-retentive and normal mice. These results indicate that both d-limonene and zingerone exhibit diuretic actions; however, the former fragrance only exerted an action in the fluid-retentive state. This different action suggests that d-limonene might be promising for the treatment of edema.

• Authors: Yasuo Morimoto, Yujiro Shibata
• Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (Yakugaku Zasshi) Vol. 130 Issue 7 Pg. 983-7 (Jul 2010) ISSN: 0031-6903 [Print] Japan
• PMID: 20606379 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Cyclohexenes
• Diuretics
• Terpenes
• zingerone
• Guaiacol
• Furosemide
• Limonene
• Deamino Arginine Vasopressin

Topics

• Animals
• Aromatherapy
• Cyclohexenes (administration & dosage, pharmacology)
• Deamino Arginine Vasopressin
• Disease Models, Animal
• Diuretics (administration & dosage)
• Dose-Response Relationship, Drug
• Edema (chemically induced, drug therapy)
• Furosemide (administration & dosage)
• Guaiacol (administration & dosage, analogs & derivatives, pharmacology)
• Limonene
• Male
• Mice
• Mice, Inbred Strains
• Terpenes (administration & dosage, pharmacology)