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Effect of malignant ascites on antioxidative potency of two tumoral cells-induced bone metastases: Walker 256/B and MatLyLu.

Abstract
This study was undertaken to estimate antioxidative status of two malignant-mammary gland carcinoma (Walker 256/B) and malignant-prostate carcinoma cells (MatLyLu) disseminated in ascitic fluids. Malignant carcinoma cells (10(7) cells) were twice serially intraperitoneal injected in male Wistar rats to develop ascites. After 7 days, ascitic fluids were collected, and cells in suspension were usable for biological assays. Cellular lipid peroxidation was assessed by measuring thiobarbituric acid reactive substances (TBARS) levels. Some antioxidant parameters: superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were also assessed. Comparisons with control baseline (cells maintained in normal culture medium) were analyzed. TBARS levels were found to be significantly decreased in both ascitic cancer cells compared to the baseline except for in the ascite I of MatLyLu cells. On the other hand, SOD and CAT activities were found to be statistically increased in the two malignant ascitic passages. GSH-Px levels were elevated in the first and in the second ascitic passages (p<0.05 and p<0.01, respectively). Our results suggest that malignant ascites are associated not only with reduced levels of TBARS but also with increased antioxidant parameters, indicating the increasing antioxidant potency of two cancer cells during malignancies process.
AuthorsR Badraoui, T Rebai
JournalExperimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (Exp Toxicol Pathol) Vol. 64 Issue 1-2 Pg. 65-8 (Jan 2012) ISSN: 1618-1433 [Electronic] Germany
PMID20605085 (Publication Type: Journal Article)
CopyrightCopyright © 2010 Elsevier GmbH. All rights reserved.
Chemical References
  • Antioxidants
  • Thiobarbituric Acid Reactive Substances
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
Topics
  • Animals
  • Antioxidants (metabolism)
  • Ascites (enzymology, metabolism, pathology)
  • Ascitic Fluid (enzymology, metabolism, pathology)
  • Bone Neoplasms (enzymology, metabolism, pathology, secondary)
  • Carcinoma 256, Walker (enzymology, metabolism, pathology)
  • Catalase (metabolism)
  • Cell Culture Techniques
  • Cell Line, Tumor
  • Cell Survival
  • Glutathione Peroxidase (metabolism)
  • Lipid Peroxidation
  • Male
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase (metabolism)
  • Thiobarbituric Acid Reactive Substances (metabolism)

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