Tectorigenin inhibits the in vitro proliferation and enhances miR-338* expression of pulmonary fibroblasts in rats with idiopathic pulmonary fibrosis.

Abstract

UNLABELLED:

Tectorigenin is one of the main components in rhizomes of Iris tectorum, which is traditionally used to treat disorders such as hepatic cirrhosis caused by fibrosis. Idiopathic pulmonary fibrosis (IPF), one of the most common interstitial lung diseases, is caused by accumulation of fibroblasts in lungs.

AIM OF THE STUDY:

In this work we sought to examine the effects of tectorigenin on pulmonary fibroblasts in the IPF animal model and investigated the molecular mechanism (microRNA regulation) of tectorigenin treatment.

MATERIALS AND METHODS:

A well-known animal disease model of pulmonary fibrosis in rat was established by intratracheally instilling of bleomycin. In vitro cultured pulmonary fibroblasts in bleomycin-treated rats and in controls were treated with or without tectorigenin. Comparative analyses of cell proliferation, apoptosis and cell cycle of pulmonary fibroblasts in bleomycin-treated rats and in controls were performed. Expression of miR-338* and its candidate gene LPA1 related to IPF of tectorigenin-treated pulmonary fibroblasts in bleomycin-treated rats were further investigated.

RESULTS:

Tectorigenin significantly inhibited the proliferation of pulmonary fibroblasts in bleomycin-treated rats but not in controls. However, no altered cell cycle and apoptosis of pulmonary fibroblasts in bleomycin-treated rats and in controls was observed after tectorigenin treatment. Tectorigenin remarkably enhanced miR-338* expression of pulmonary fibroblasts in bleomycin-treated rats and downregulated LPA1 in the protein level.

CONCLUSIONS:

Tectorigenin inhibits the proliferation of pulmonary fibroblasts in vitro and enhances miR-338* expression, which might in turn downregulate LPA1. This indicates a potential inhibitory role of tectorigenin on the pathogenesis of IPF.

• Authors: Huan Zhang, Xiufang Liu, Shi Chen, Junhua Wu, Xie Ye, Lizhi Xu, Huimei Chen, Deping Zhang, Renxiang Tan, Yaping Wang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 131 Issue 1 Pg. 165-73 (Aug 19 2010) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 20600766 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2010 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Growth Inhibitors
• Isoflavones
• MIRN338 microRNA, human
• MIRN338 microRNA, rat
• MicroRNAs
• tectorigenin

Topics

• Animals
• Cell Proliferation (drug effects)
• Cells, Cultured
• Disease Models, Animal
• Fibroblasts (drug effects, metabolism, pathology)
• Gene Expression Regulation (drug effects)
• Growth Inhibitors (pharmacology)
• Isoflavones (pharmacology, therapeutic use)
• Lung (cytology, drug effects, metabolism)
• Male
• MicroRNAs (biosynthesis, genetics)
• Pulmonary Fibrosis (drug therapy, genetics, pathology)
• Rats
• Rats, Sprague-Dawley