Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation.

Abstract

Multifunctional poly(glycidyl methacrylate) (PGMA) microspheres containing magnetic, fluorescent, and cancer cell-specific moieties were prepared in four steps: (i) preparation of parent PGMA microspheres by dispersion polymerization and their reaction with ethylenediamine to obtain amino groups, (ii) precipitation of iron ions (Fe(2+) and Fe(3+)) to form Fe(3)O(4) nanoparticles within the microspheres, (iii) consecutive reactions of folic acid with the amino groups on PGMA, and (iv) incorporation of fluorescein isothiocyanate into the microspheres. The microspheres were superparamagnetic, highly monodispersive, intensively fluorescent, and capable of recognizing and binding cancer cells that overexpress folic acid receptors. It was demonstrated that with these microspheres, HeLa cells could be captured from their suspension and easily moved in the direction of the externally applied magnetic field.

• Authors: Xiaoqing Dong, Yonghui Zheng, Yubin Huang, Xuesi Chen, Xiabin Jing
• Journal: Analytical biochemistry (Anal Biochem) Vol. 405 Issue 2 Pg. 207-12 (Oct 15 2010) ISSN: 1096-0309 [Electronic] United States
• PMID: 20599531 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Inc. All rights reserved.

Chemical References

• Polymethacrylic Acids
• polyglycidyl methacrylate
• Folic Acid

Topics

• Cell Separation (methods)
• Folic Acid (chemistry, metabolism)
• HeLa Cells
• Humans
• Microspheres
• Nanoparticles (chemistry)
• Polymerization
• Polymethacrylic Acids (chemistry)