Structural characterization of laminaran and galactofucan extracted from the brown seaweed Saccharina longicruris.

Abstract

Brown seaweed contains several polysaccharides like laminaran, fucoidan and alginate. Laminaran is a beta-glucan that has shown anti-apoptotic and anti-tumoral activities, while galactofucan (fucoidan) is a sulfated polysaccharide that has displayed anticoagulant, anti-tumor, anti-thrombosis, anti-inflammatory and antiviral properties. In this study, crude laminaran and galactofucan (fucoidan) were extracted from the brown seaweed Saccharina longicruris at four harvest periods (M05, A05, N05 and J06). The galactofucan M05 and N05 fractions were depolymerized (RDP) over 2 or 4h to give 4 RDP fractions (M05 RDP 2H, M05 RDP 4H, N05 RDP 2H and N05 RDP 4H) whose molecular weights, monosaccharide compositions and glycosidic linkages were determined by GC-MS. The laminaran fraction gave a molecular weight range from 2900 to 3300 Da and contained between 50.6% and 68.6% d-glucose and an average of 1.3% D-mannitol. The presence of a beta-(1,3) linkage between D-glucose in the main chain was observed, with branching at positions 6 and 2. The M05 fraction contained less branching than other laminaran fractions, which might have influenced its conformation in solution and thus its activity. The crude galactofucan fractions displayed a molecular weight range from 638 to 1529 kDa, whereas the RDP fractions had molecular weights <30 kDa. The structure of the galactofucan fractions remained complex after depolymerization, with these also being more sulfated (30-39%) than the crude fractions (13-20%). The crude and RDP fractions contained 3-linked fucopyranose 4-sulfate and 6-linked galactopyranose 3-sulfate moieties, although the galactofucans isolated from M05 and J06 contained less 6-linked galactopyranose 3-sulfate than the A05 and N05 fractions.

• Authors: Laurie-Eve Rioux, Sylvie L Turgeon, Martin Beaulieu
• Journal: Phytochemistry (Phytochemistry) Vol. 71 Issue 13 Pg. 1586-95 (Sep 2010) ISSN: 1873-3700 [Electronic] England
• PMID: 20599236 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Glucans
• Polysaccharides
• laminaran
• fucoidan

Topics

• Gas Chromatography-Mass Spectrometry
• Glucans
• Polysaccharides (chemistry, isolation & purification)
• Seaweed (chemistry)
• Spectrometry, Mass, Electrospray Ionization
• Spectroscopy, Fourier Transform Infrared
• Time Factors