Abstract | BACKGROUND: METHODS: Mice were randomly assigned to one of the following groups: (1) sham-operated; (2) sham+HB- EGF (1200 microg/kg in 0.6 mL administered by intra- luminal injection at the jejuno-ileal junction immediately after identification of the superior mesenteric artery); (3) superior mesenteric artery occlusion for 45 min followed by reperfusion for 6 h (I/R); or (4) I/R+HB- EGF (1200 microg/kg in 0.6 mL) administered 15 min after vascular occlusion. The severity of acute lung injury was determined by histology, morphometric analysis and invasive pulmonary function testing. Animal survival was evaluated using Kaplan-Meier analysis. RESULTS: Mice subjected to intestinal I/R injury showed histologic and functional evidence of acute lung injury and decreased survival compared with sham-operated animals. Compared with mice treated with HB-EGF (I/R+HB- EGF), the I/R group had more severe acute lung injury, and decreased survival. CONCLUSION:
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Authors | Iyore A O James, Chun-Liang Chen, Guangcun Huang, Hong-Yi Zhang, Markus Velten, Gail E Besner |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 163
Issue 1
Pg. 86-95
(Sep 2010)
ISSN: 1095-8673 [Electronic] United States |
PMID | 20599214
(Publication Type: Journal Article)
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Copyright | Copyright 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Hbegf protein, mouse
- Heparin-binding EGF-like Growth Factor
- Intercellular Signaling Peptides and Proteins
- Proto-Oncogene Proteins c-akt
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Topics |
- Animals
- Apoptosis
(drug effects)
- Capillary Permeability
(drug effects)
- Heparin-binding EGF-like Growth Factor
- Intercellular Signaling Peptides and Proteins
(pharmacology, therapeutic use)
- Intestine, Small
(blood supply)
- Lung
(blood supply, drug effects)
- Lung Injury
(etiology, metabolism, prevention & control)
- Male
- Mice
- Mice, Inbred C57BL
- Neutrophil Infiltration
(drug effects)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Pulmonary Diffusing Capacity
(drug effects)
- Reperfusion Injury
(complications)
- Respiratory Function Tests
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