Effects of intestinal intervention on bacterial translocation in a rat model of acute liver failure in vivo.
Abstract | BACKGROUND/AIMS: METHODS: D- Galactosamine was injected into the abdominal cavity of rats to induce ALF. Escherichia coli JM109 labeled with enhanced green fluorescent protein was administered to track BT. Simultaneously, the rats were given lactulose or smecta. Blood samples were collected for measuring liver function, cytokines, endotoxins, and TLR4 expression. Representative tissue specimens from the liver, spleen, and mesenteric lymph nodes were aseptically harvested for bacterial identification by agarose gel electrophoresis, laser scanning confocal microscopy, and flow cytometry. RESULTS: BT occurred in ALF, accompanied by impaired liver function with increased cytokines, endotoxins, and TLR4 expression. After the treatment with lactulose or smecta, all these parameters decreased, including the relative quantity of translocated bacteria while albumin increased. Furthermore, compared with the lactulose treatment group, the parameters in the smecta treatment group improved. Moreover, in the group in which smecta was given for prophylaxis, there was greater improvement than with treatment. CONCLUSION:
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Authors | Jie Yang, Qing-He Nie, An-Hui Wang, Xiao-Feng Huang, Qing-Quan Liu, Yun-Ming Li |
Journal | European journal of gastroenterology & hepatology
(Eur J Gastroenterol Hepatol)
Vol. 22
Issue 11
Pg. 1316-22
(Nov 2010)
ISSN: 1473-5687 [Electronic] England |
PMID | 20595903
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Endotoxins
- Gastrointestinal Agents
- Silicates
- Tlr4 protein, rat
- Toll-Like Receptor 4
- enhanced green fluorescent protein
- smecta
- Bentonite
- Green Fluorescent Proteins
- Lactulose
- Galactosamine
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Topics |
- Animals
- Bacterial Translocation
(drug effects)
- Bentonite
(pharmacology)
- Cytokines
(blood)
- Disease Models, Animal
- Electrophoresis, Agar Gel
- Endotoxins
(blood)
- Escherichia coli
(drug effects, genetics, metabolism)
- Flow Cytometry
- Galactosamine
- Gastrointestinal Agents
(therapeutic use)
- Green Fluorescent Proteins
(biosynthesis, genetics)
- Intestines
(drug effects, immunology, microbiology)
- Lactulose
(pharmacology)
- Liver
(drug effects, microbiology, pathology)
- Liver Failure, Acute
(chemically induced, drug therapy, immunology, microbiology)
- Lymph Nodes
(drug effects, microbiology)
- Male
- Microscopy, Confocal
- Rats
- Rats, Sprague-Dawley
- Silicates
(pharmacology)
- Spleen
(drug effects, microbiology)
- Toll-Like Receptor 4
(blood)
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