Expression of the carbohydrate tumor marker Sialyl Lewis a (Ca19-9) in squamous cell carcinoma of the larynx.

Abstract

BACKGROUND:

The clinical relevance of the carbohydrate antigen Sialyl Lewis a (SLea) as a serum tumor marker in diagnosis and follow-up treatment is unquestioned in a broad spectra of human carcinomas. Overexpression of this antigen is combined with poor prognosis and malignant relapse. The aim of our study was the systematic investigation of SLea expression in squamous cell carcinoma of the larynx versus normal and phlogistic tissue.

MATERIALS AND METHODS:

Paraffin-embedded sections of normal, phlogistic and squamous cell carcinoma tissue were incubated with a monoclonal antibody against SLea. The staining reaction was performed using ABC-Peroxidase and DAB. As a positive control tissue of breast cancer was used and the negative control was performed with unspecific mouse IgM. Semiquantitative evaluations were carried out double-blinded by two independent investigators, including a pathologist.

RESULTS:

A very faint expression of SLea (Ca19-9) in normal laryngeal tissue, a moderate upregulation in phlogistic tissue and a dramatic upregulation in some types of squamous cell carcinoma of the larynx were observed. Laryngeal cancer is the most common cancer of the upper aerodigestive tract. Most cases of laryngeal cancer are squamous cell carcinoma and can be classified into: well differentiated (more than 75% keratinization), moderately differentiated (25-75% keratinization), and poorly differentiated (<25% keratinisation) carcinomas.

CONCLUSION:

The results of this study indicate that SLea is a potential tumor marker in carcinoma of the larynx.

• Authors: Irmi Wiest, Christoph Alexiou, Doris Mayr, Sandra Schulze, Christina Kuhn, Susanne Kunze, Mareike Büttner, Peter Betz, Udo Jeschke
• Journal: Anticancer research (Anticancer Res) Vol. 30 Issue 5 Pg. 1849-53 (May 2010) ISSN: 1791-7530 [Electronic] Greece
• PMID: 20592391 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• CA-19-9 Antigen
• Carbohydrates
• Lewis X Antigen
• Sialyl Lewis X Antigen
• Keratins

Topics

• Biomarkers, Tumor (biosynthesis)
• CA-19-9 Antigen (biosynthesis)
• Carbohydrates (chemistry)
• Carcinoma, Squamous Cell (blood)
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry (methods)
• Keratins (chemistry)
• Laryngeal Neoplasms (blood)
• Lewis X Antigen (biosynthesis)
• Sialyl Lewis X Antigen
• Up-Regulation