Abstract | BACKGROUND/AIMS: Mitochondrial permeability transition has a critical role in ischemia/reperfusion (I/R)-induced kidney injury. It is thought that mitochondrial permeability transition occurs after the opening of the permeability transition pore, a channel which putatively consists of a voltage-dependent anion channel, adenine nucleotide translocator and cyclophilin D (CypD). Much evidence shows that CypD plays an important role in I/R-induced injury. METHODS: To evaluate the role of CypD following I/R renal injury, we tested the hypothesis that knockdown of CypD gene by RNA interference (RNAi) protects rat from I/R-induced renal injury. RESULTS: Our data show that knockdown of CypD by RNAi protects normal rat kidney cell line from hypoxia-induced necrotic death. Infection of lentivirus expressing CypD RNAi sequence produces a significant reduction of CypD at both mRNA and protein levels. Both pathologic and biochemical analyses show that knockdown of CypD by RNAi protects rat kidney from I/R-induced renal injury. CONCLUSION: Our study provides the evidence that CypD may be a potential target for protecting I/R-induced renal injury.
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Authors | Wei Hu, Zhiqiang Chen, Zhangqun Ye, Ding Xia, Zhongyu Xia, Jiefeng Ma, Min Zhu, Gang Chen |
Journal | Kidney & blood pressure research
(Kidney Blood Press Res)
Vol. 33
Issue 3
Pg. 193-9
( 2010)
ISSN: 1423-0143 [Electronic] Switzerland |
PMID | 20588055
(Publication Type: Journal Article)
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Copyright | Copyright © 2010 S. Karger AG, Basel. |
Chemical References |
- Cyclophilin D
- Cyclophilins
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Topics |
- Animals
- Cell Hypoxia
(genetics)
- Cell Line
- Cyclophilin D
- Cyclophilins
(deficiency, genetics)
- Gene Knockdown Techniques
(methods)
- Kidney
(blood supply, metabolism)
- Male
- RNA Interference
(physiology)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(genetics, metabolism, prevention & control)
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