Abstract |
Isolated perfused rat lungs were subjected to oxidant injury induced by tert-butyl hydroperoxide (t-buOOH), which caused a significant increase in capillary permeability as assessed by the change in the capillary filtration coefficient. t-buOOH caused an increase in the change in the capillary filtration coefficient (delta Kfc) of 0.27 +/- 0.05 ml.min.cmH2O-1.100 g lung tissue-1 (mean +/- SE) that was accompanied by an increase in thiobarbituric acid reactive products of lipid peroxidation in the lung perfusate. The addition of hemoglobin to the perfusate potentiated t-buOOH-induced lung injury as evidenced by a significantly greater (P = 0.007) delta Kfc of 0.43 +/- 0.05. t-buOOH also caused hemoglobin to release large quantities of free iron in vitro. The potentiation of t-buOOH-induced lung injury by hemoglobin was prevented by apotransferrin as evidenced by a significant reduction (P = 0.001) in delta Kfc to 0.13 +/- 0.02. No statistically significant (P greater than 0.05) changes in segmental resistances or pulmonary vascular pressures occurred in any of the lungs injured with t-buOOH when compared with time controls. These results demonstrate that t-buOOH causes an oxidant injury in isolated rat lungs that can be potentiated by free iron released from hemoglobin.
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Authors | A F Seibert, A E Taylor, J B Bass, J Haynes Jr |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 260
Issue 6 Pt 2
Pg. H1980-4
(Jun 1991)
ISSN: 0002-9513 [Print] United States |
PMID | 2058730
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Apoproteins
- Fenton's reagent
- Hemoglobins
- Membrane Lipids
- Peroxides
- Thiobarbiturates
- Transferrin
- apotransferrin
- tert-Butylhydroperoxide
- Hydrogen Peroxide
- Iron
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Topics |
- Animals
- Apoproteins
(physiology)
- Capillary Permeability
(drug effects)
- Hemodynamics
(drug effects)
- Hemoglobins
(metabolism, physiology)
- Hydrogen Peroxide
(metabolism)
- In Vitro Techniques
- Iron
(metabolism)
- Lipid Peroxidation
- Lung
(drug effects, metabolism)
- Male
- Membrane Lipids
(metabolism)
- Oxidation-Reduction
- Peroxides
(adverse effects)
- Rats
- Thiobarbiturates
(metabolism)
- Transferrin
(physiology)
- tert-Butylhydroperoxide
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