[Role of endogenous porphyrins in laser therapy of experimental skin wounds].

Abstract

The role of endogenous porphyrins in the effect of laser irradiation on the superoxide dismutase (SOD) activity of wound exudate and rat leukocyte activity has been studied on models of aceptic incised skin wounds. Wounds were irradiated by a He-Ne laser (632.8 nm, 1.5 J/cm2) on the 2nd, 3rd, and 4th days after the beginning of the experiment. Irradiation effects were evaluated by the SOD activity (NBT test) and the activity of leukocytes of wound exudate (as a chemiluminescent response to opsonized zymosan). It was found that in animals subjected to laser irradiation, the SOD activity sharply increased. This effect depended on endogenous porphyrin concentration and was retained throughout the experiment. The SOD activity in unirradiated animals decreased from the 2nd to the 5th day of experiment. The evaluation of the activity of wound exudate leukocytes did not reveal any distrinct dependence of the effect on the concentration of endogenous porphyrins.

• Authors: T V Machneva, N N Bulgakova, Iu A Vladimirov, A N Osipov
• Journal: Biofizika (Biofizika) 2010 May-Jun Vol. 55 Issue 3 Pg. 532-8 ISSN: 0006-3029 [Print] Russia (Federation)
• PMID: 20586335 (Publication Type: English Abstract, Journal Article)

Chemical References

• Porphyrins
• Superoxide Dismutase

Topics

• Animals
• Exudates and Transudates (immunology, metabolism)
• Lasers, Gas (therapeutic use)
• Leukocytes (pathology)
• Low-Level Light Therapy
• Male
• Porphyrins (metabolism)
• Rats
• Skin (injuries, metabolism, radiation effects)
• Spectrometry, Fluorescence
• Superoxide Dismutase (metabolism)