Abstract | BACKGROUND AND PURPOSE: METHODS AND MATERIALS: We used various cell lines deficient in non-homologous end-joining (NHEJ) or homologous recombination (HR) as well as DLD-1 and HeLa in clonogenic assay. gamma-H2AX focus formation and SCneo assay was performed to examine the effects of Gimeracil on DNA double strand break ( DSB) repair mechanisms. RESULTS: Results of gamma-H2AX focus assay indicated that Gimeracil inhibited DNA DSB repair. It did not sensitize cells deficient in HR but sensitized those deficient in NHEJ. In SCneo assay, Gimeracil reduced the frequency of neo-positive clones. Additionally, it sensitized the cells in S-phase more than in G0/G1. CONCLUSIONS:
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Authors | Masaru Takagi, Koh-ichi Sakata, Masanori Someya, Hiroshi Tauchi, Kenta Iijima, Yoshihisa Matsumoto, Toshihiko Torigoe, Akari Takahashi, Masato Hareyama, Masakazu Fukushima |
Journal | Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology
(Radiother Oncol)
Vol. 96
Issue 2
Pg. 259-66
(Aug 2010)
ISSN: 1879-0887 [Electronic] Ireland |
PMID | 20584556
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Pyridines
- gimeracil
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Topics |
- Antineoplastic Agents
(pharmacology)
- Cell Line
- Cell Line, Tumor
- Flow Cytometry
- Humans
- Pyridines
(pharmacology)
- Radiation Tolerance
(drug effects)
- Recombination, Genetic
(drug effects)
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