Tumor suppressor QM-like gene from disk abalone (Haliotis discus discus): molecular characterization and transcriptional analysis upon immune challenge.

Abstract

We describe molecular characterization and transcriptional analysis of the gene encoding tumor suppressor QM-like protein, AbQM, in the disk abalone Haliotis discus discus. The full-length cDNA (765-bp) of AbQM was found to consist of a 654-bp ORF coding for a 218 amino acid protein of a 25 kDa molecular mass with a 10.2 isoelectric point. Analysis of AbQM sequence revealed the presence of characteristic motifs, including the ribosomal protein L10 signature, SH3-binding motif and two antibiotic binding sites. Phylogenetic analysis confirmed that AbQM is closely related to other mollusk QM proteins, and altogether they form a mollusk QM protein sub-family which displays evolutionary conservation from yeast to human. Tissue-specific expression and transcriptional regulation of AbQM was analyzed by quantitative real-time PCR in response to bacterial (Vibrio alginolyticus and Vibrio parahemolyticus, Listeria monocytogenes) and viral (viral hemorrhagic septicemia virus, VHSV) challenge. AbQM transcripts were found to be expressed ubiquitously in all examined tissues in a constitutive manner, as similar expression levels were detected in hemocytes, mantle, digestive tract and muscle. Upon bacterial and VHSV challenge, AbQM showed significant up-regulation in gills, but not in hemocytes. Taken together, these findings suggest that AbQM in abalone-like mollusks can respond to and facilitate a defensive effect against pathogenic infection.

• Authors: Chulhong Oh, Mahanama De Zoysa, Chamilani Nikapitiya, Ilson Whang, Yu Cheol Kim, Do-Hyung Kang, Soo-jin Heo, Young-Ung Choi, Cheol Young Choi, Jae-Seong Lee, Jehee Lee
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 29 Issue 3 Pg. 494-500 (Sep 2010) ISSN: 1095-9947 [Electronic] England
• PMID: 20580829 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Ltd. All rights reserved.

Chemical References

• RPL10 protein, human
• Ribosomal Proteins

Topics

• Amino Acid Sequence
• Animals
• Bacteria (immunology)
• Base Sequence
• Gastropoda (classification, genetics, immunology, microbiology)
• Gene Expression Profiling
• Gene Expression Regulation (immunology)
• Immunity, Innate (immunology)
• Molecular Sequence Data
• Novirhabdovirus (physiology)
• Phylogeny
• Ribosomal Protein L10
• Ribosomal Proteins (genetics, immunology)
• Sequence Alignment