Granulocyte antibody screening: evaluation of a bead-based assay in comparison with classical methods.

Abstract

BACKGROUND:

Granulocyte antibodies have been implicated in allo- and autoimmune neutropenia and in transfusion reactions.

STUDY DESIGN AND METHODS:

Fifty-one sera from suspected alloimmune neutropenia or transfusion-related acute lung injury (TRALI) and 40 sera from suspected autoimmune neutropenia were tested for granulocyte antibodies using LABScreen MULTI (One Lambda, Inc.), compared with classical tests (flow cytometry [FC] and granulocyte agglutination [GAT] followed by monoclonal antibody-specific immobilization of granulocyte antigens [MAIGA]).

RESULTS:

In alloimmune situations, 48 sera were concordant (94%), two sera positive for HNA with LABScreen MULTI were negative by FC/GAT and/or MAIGA, and one serum sample negative for HNA with LABScreen MULTI was positive by classical tests. In autoimmune neutropenia, 30 sera were concordant (75%), four sera positive for HNA with LABScreen MULTI were negative by FC/GAT and/or MAIGA, and six sera negative for HNA with LABScreen MULTI were positive by FC/GAT and/or MAIGA. For detection of autoantibodies, the LABScreen MULTI was less concordant. However, with the exception of one case, the discrepancies were observed in sera that did not show a clear specificity.

CONCLUSIONS:

LABScreen MULTI correlated well with our classical methods for HNA-1 and HNA-2a antibody screening. It can be used for screening blood donors or patients suspected of TRALI, but GAT is still needed for HNA-3a antibody screening.

• Authors: Patricia Fromont, Nolwen Prié, Philippe Simon, Anne Cesbron-Gautier, Erwann Quelvennec, Jean-Denis Bignon, Jean-Yves Muller, Marie Audrain
• Journal: Transfusion (Transfusion) Vol. 50 Issue 12 Pg. 2643-8 (Dec 2010) ISSN: 1537-2995 [Electronic] United States
• PMID: 20576014 (Publication Type: Comparative Study, Evaluation Study, Journal Article)
• Copyright: © 2010 American Association of Blood Banks.

Chemical References

• Antibodies
• Autoantibodies
• Isoantigens

Topics

• Acute Lung Injury (blood, etiology, immunology)
• Antibodies (analysis, blood, immunology)
• Autoantibodies (analysis, immunology)
• Granulocytes (immunology)
• Humans
• Immunoassay (methods, standards)
• Immunomagnetic Separation (methods, standards)
• Infant, Newborn
• Isoantigens (analysis, immunology)
• Mass Screening (methods, standards)
• Neutropenia (blood, congenital, immunology)
• Reference Values
• Serologic Tests (methods, standards)
• Thrombocytopenia, Neonatal Alloimmune (blood, immunology)
• Transfusion Reaction