Quantitative and qualitative assay of rubella IgA antibody in breast milk.
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| Abstract |
Breast milk contains immunological factors, such as IgA antibody, which help to prevent infectious diseases. A total of 197 paired samples of colostrum and breast milk was collected from postpartum mothers in Gunma City, Japan, and examined for anti-rubella IgA antibody by enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB). The anti-rubella virus IgA ranged from 0.5 to 78.5 U/ml with a mean of 6.05 U/ml and a median of 3.6 U/ml in colostrum, and from 0.5 to 32.7 U/ml with a mean of 2.74 U/ml and a median of 2 U/ml in milk. The differences between the means of titers of total IgA and anti-rubella virus IgA in colostrum and in milk were significant statistically. The levels of anti-rubella virus IgA in both colostrum and breast milk correlated positively with the anti-rubella virus hemagglutination inhibition (HI) titers in the sera of mother, indicating that the levels of these different classes of antibodies correlated. Based on WB, anti-rubella virus IgA in both colostrum and breast milk reacted with the rubella viral protein E1 and C, but not with the E2 protein.
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| Authors | Yuko Hayakawa, Yumei Zhou, Masashi Mizuguchi, Teryl K Frey, Hiroshi Ushijima |
| Journal | Journal of medical virology (J Med Virol) Vol. 82 Issue 8 Pg. 1475-9 (Aug 2010) ISSN: 1096-9071 [Electronic] United States |
| PMID | 20572092 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | (c) 2010 Wiley-Liss, Inc. |
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