Abstract |
The aim of this study was to evaluate the anti- tumor activity of Amblyomin-X, a serine protease Kunitz-type inhibitor. Amblyomin-X induced tumor mass regression and decreased number of metastatic events in a B16F10 murine melanoma model. Alterations on expression of several genes related to cell cycle were observed when two tumor cell lines were treated with Amblyomin-X. PSMB2, which encodes a proteasome subunit, was differentially expressed, in agreement to inhibition of proteasomal activity in both cell lines. In conclusion, our results indicate that Amblyomin-X selectively acts on tumor cells by inducing apoptotic cell death, possibly by targeting the ubiquitin- proteasome system.
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Authors | Ana Marisa Chudzinski-Tavassi, Paulo Luis De-Sá-Júnior, Simone Michaela Simons, Durvanei Augusto Maria, Janaina de Souza Ventura, Isabel de Fátima Correia Batista, Fernanda Faria, Esther Durães, Eduardo Moraes Reis, Marilene Demasi |
Journal | Toxicon : official journal of the International Society on Toxinology
(Toxicon)
Vol. 56
Issue 7
Pg. 1145-54
(Dec 15 2010)
ISSN: 1879-3150 [Electronic] England |
PMID | 20570593
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Amblyomin-X protein, Amblyomma cajennense
- Antineoplastic Agents
- Arthropod Proteins
- Salivary Proteins and Peptides
- Serine Proteinase Inhibitors
- Ubiquitin
- Proteasome Endopeptidase Complex
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Topics |
- Animals
- Antineoplastic Agents
(chemistry, isolation & purification, therapeutic use)
- Apoptosis
(drug effects)
- Arthropod Proteins
- Cell Cycle
(drug effects)
- Cell Line, Tumor
- Disease Models, Animal
- Gene Expression Regulation, Neoplastic
(drug effects)
- Ixodidae
(chemistry, genetics)
- Melanoma, Experimental
(drug therapy, pathology)
- Mice
- Mice, Inbred C57BL
- Neoplasm Metastasis
- Proteasome Endopeptidase Complex
(drug effects, metabolism)
- Salivary Glands
(chemistry)
- Salivary Proteins and Peptides
(chemistry, isolation & purification, therapeutic use)
- Serine Proteinase Inhibitors
(chemistry, isolation & purification, therapeutic use)
- Ubiquitin
(metabolism)
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