Identification of a 2-cell stage specific inhibitor of the cleavage of preimplantation mouse embryos synthesized by rat hepatoma cells as 5'-deoxy-5'-methylthioadenosine.

Abstract

Rat hepatoma Reuber H-35 cells produce a unique compound designated as Fr.B-25, a 2-cell stage-specific inhibitor of the cleavage of preimplantation mouse embryos cultured in vitro. Here, we identified Fr.B-25 as a purine nucleoside, 5'-deoxy-5'-methylthioadenosine (MTA), by mass spectroscopic analysis. All of the biological activities examined of authentic MTA on the development of mouse zygotes were indistinguishable from those of Fr.B-25. The mechanism of MTA action in the development of preimplantation mouse embryos was probably different from those of hypoxanthine and adenosine, which are well-characterized purine nucleosides that act as inhibitors of the cleavage of mouse 2-cell embryos. From the shared molecular and biological properties of Fr.B-25 and MTA, we concluded that Fr.B-25 is MTA. To the best of our knowledge, this is the first delineation of the effect of MTA on the development of preimplantation mammalian embryos cultured in vitro.

• Authors: Masayuki Kobayashi, Koichi Saito, Shigeru Tamogami, Junko Takashima, Kano Kasuga, Ikuo Kojima
• Journal: Zygote (Cambridge, England) (Zygote) Vol. 19 Issue 2 Pg. 117-25 (May 2011) ISSN: 1469-8730 [Electronic] England
• PMID: 20569511 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Deoxyadenosines
• Thionucleosides
• 5'-methylthioadenosine

Topics

• Animals
• Blastocyst (drug effects, metabolism)
• Cleavage Stage, Ovum (cytology, metabolism)
• Deoxyadenosines (biosynthesis, chemistry, pharmacology)
• Embryo, Mammalian (metabolism)
• Embryonic Development
• Female
• Liver Neoplasms, Experimental (metabolism)
• Mice
• Rats
• Thionucleosides (biosynthesis, chemistry, pharmacology)