Isorhamnetin-3-glucoside alleviates oxidative stress and opacification in selenite cataract in vitro.

Abstract

Oxidative stress has long been recognized as an important mediator in the pathogenesis of cataract and the goal of this study was to determine the efficacy of isorhamnetin-3-glucoside (IR3G) in alleviating the toxicity induced by sodium selenite in in vitro culture condition. IR3G is the bioactive flavonoid isolated and characterized from the leaves of Cochlospermum religiosum. Enucleated rat lenses were maintained in organ culture containing M-199 medium alone (G-I), supplemented with 0.1 mM selenite (G-II) and selenite + 25 microg/ml IR3G (G-III). Treatment to G-III was from the second to fifth day while selenite administration to G-II & III was done on the third day. The antioxidant potential of the compound was assessed by Cu(2+) induced lipoprotein diene formation and superoxide scavenging assays. Morphological examination of the lenses also gave a supporting data. Antioxidant enzymes-superoxide dismutase (SOD), catalase and concentration of reduced glutathione (GSH) were significantly lower, while TBARS showed an increase in G-II than that in G-III and G-I lenses. Activity of Ca(2+)-ATPase was decreased and level of calcium was increased in G-II than G-III and G-I lenses. These data suggest that IR3G is able to significantly retard selenite cataract in vitro by virtue of its antioxidant property.

• Authors: V Gayathri Devi, B N Rooban, V Sasikala, V Sahasranamam, Annie Abraham
• Journal: Toxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 24 Issue 6 Pg. 1662-9 (Sep 2010) ISSN: 1879-3177 [Electronic] England
• PMID: 20566334 (Publication Type: Journal Article)
• Copyright: Copyright 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Antioxidants
• Drug Combinations
• Flavonols
• Plant Extracts
• Thiobarbituric Acid Reactive Substances
• isorhamnetin 3-O-glucoside
• Catalase
• Superoxide Dismutase
• Glutathione
• Sodium Selenite
• Copper Sulfate

Topics

• Animals
• Antioxidants (pharmacology)
• Bixaceae (chemistry)
• Catalase (metabolism)
• Cataract (drug therapy, metabolism)
• Copper Sulfate (pharmacology)
• Drug Combinations
• Female
• Flavonols (pharmacology)
• Glutathione (metabolism)
• Lens, Crystalline (drug effects)
• Organ Culture Techniques
• Oxidative Stress (drug effects)
• Plant Extracts (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Sodium Selenite (toxicity)
• Superoxide Dismutase (metabolism)
• Thiobarbituric Acid Reactive Substances (metabolism)