Abstract |
Nanotechnology is an innovative method of freely controlling nanometre-sized materials. Recent outbreaks of mucosal infectious diseases have increased the demands for development of mucosal vaccines because they induce both systemic and mucosal antigen-specific immune responses. Here we developed an intranasal vaccine-delivery system with a nanometre-sized hydrogel (' nanogel') consisting of a cationic type of cholesteryl-group-bearing pullulan (cCHP). A non-toxic subunit fragment of Clostridium botulinum type-A neurotoxin BoHc/A administered intranasally with cCHP nanogel (cCHP-BoHc/A) continuously adhered to the nasal epithelium and was effectively taken up by mucosal dendritic cells after its release from the cCHP nanogel. Vigorous botulinum-neurotoxin-A-neutralizing serum IgG and secretory IgA antibody responses were induced without co-administration of mucosal adjuvant. Importantly, intranasally administered cCHP-BoHc/A did not accumulate in the olfactory bulbs or brain. Moreover, intranasally immunized tetanus toxoid with cCHP nanogel induced strong tetanus-toxoid-specific systemic and mucosal immune responses. These results indicate that cCHP nanogel can be used as a universal protein-based antigen-delivery vehicle for adjuvant-free intranasal vaccination.
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Authors | Tomonori Nochi, Yoshikazu Yuki, Haruko Takahashi, Shin-ichi Sawada, Mio Mejima, Tomoko Kohda, Norihiro Harada, Il Gyu Kong, Ayuko Sato, Nobuhiro Kataoka, Daisuke Tokuhara, Shiho Kurokawa, Yuko Takahashi, Hideo Tsukada, Shunji Kozaki, Kazunari Akiyoshi, Hiroshi Kiyono |
Journal | Nature materials
(Nat Mater)
Vol. 9
Issue 7
Pg. 572-8
(Jul 2010)
ISSN: 1476-4660 [Electronic] England |
PMID | 20562880
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Gels
- Vaccines
- Botulinum Toxins, Type A
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Topics |
- Administration, Intranasal
- Animals
- Botulinum Toxins, Type A
(chemistry)
- Brain
(metabolism)
- Clostridium botulinum
(metabolism)
- Drug Delivery Systems
- Female
- Gels
(chemistry)
- Immune System
- Materials Testing
- Mice
- Mice, Inbred BALB C
- Nanoparticles
(chemistry)
- Nanotechnology
(methods)
- Olfactory Bulb
(metabolism)
- Vaccines
(chemistry)
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