KIOM-79 prevents xylose-induced lens opacity and inhibits TGF-beta2 in human lens epithelial cells cultured under high glucose.

Abstract

AIM OF THE STUDY:

To investigate the effects of KIOM-79 in preventing the development of diabetic complications, such as cataracts.

MATERIALS AND METHODS:

The inhibitory effects of KIOM-79 were assessed in a model of xylose-induced lens opacity and on changes mediated by high levels of glucose in human lens epithelial (HLE-B3) cells.

RESULTS:

In lenses treated with KIOM-79, opacity was significantly improved and glutathione (GSH) was increased compared to controls. In HLE-B3 cells treated with KIOM-79, high glucose-mediated increases in TGF-beta2, alphaB-crystallin, and fibronectin were significantly inhibited in a dose-dependent manner. KIOM-79 decreased the phosphorylation of p-Smad2/3, pp38MAPK, pp44/42, and NF-kappaB signaling in cells grown under high glucose conditions.

CONCLUSION:

KIOM-79 is protective against lens opacity and protects HLE-B3 cells from the toxic effects of high glucose. Therefore, KIOM-79 may provide a potential therapeutic approach for preventing diabetic complications, such as cataracts.

• Authors: Nan Hee Kim, Young Sook Kim, Dong Ho Jung, Jin Sook Kim
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 130 Issue 3 Pg. 599-606 (Aug 09 2010) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 20561927 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright 2010. Published by Elsevier Ireland Ltd. All rights reserved.

Chemical References

• KIOM 79
• Plant Extracts
• Protective Agents
• Transforming Growth Factor beta2
• Xylose
• Glutathione
• Glucose

Topics

• Cell Line
• Corneal Opacity (prevention & control)
• Dose-Response Relationship, Drug
• Epithelial Cells (drug effects, metabolism)
• Glucose (metabolism)
• Glutathione (drug effects, metabolism)
• Humans
• Lens, Crystalline (cytology, drug effects)
• Plant Extracts (administration & dosage, pharmacology)
• Protective Agents (administration & dosage, pharmacology)
• Signal Transduction (drug effects)
• Transforming Growth Factor beta2 (antagonists & inhibitors)
• Xylose (toxicity)