Abstract | PURPOSE: METHODS: This retrospective case-control study included 26 laboratory-confirmed cases of ocular toxoplasmosis (group A), 41 clinically suspected cases (group B), and 67 currently healthy blood donors who were chronically infected with T. gondii (group C). Laboratory and clinical data were analyzed according to the ocular presentation and Goldmann-Witmer's coefficient. Serum and aqueous humor were sampled at the time of uveitis. Serum anti-Hsp70.1 antibody levels were obtained by ELISA. The probability of ocular toxoplasmosis was estimated by a logistic regression analysis that combined data from serum IgG anti-Hsp70.1 and aqueous-humor IgG anti-T. gondii antibody levels. RESULTS: Serum IgG anti-Hsp70.1 antibody levels were significantly increased in groups A and B when compared to the levels in control group C (P ≤ 0.0034). These levels correlated with the retinal lesion size (r = 0.301; P < 0.0349). Logistic probability and anti-Hsp70.1 antibodies in sera confirmed that 10 of 23 cases in group B were true ocular toxoplasmosis. CONCLUSIONS: Anti-Hsp70 may play a role in the immunopathogenesis of ocular Toxoplasma infection. This study showed that the anti-Hsp70.1 antibody and the logistic probability test can confirm clinically suspected ocular toxoplasmosis.
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Authors | Bernabé F F Chumpitazi, Laurence Bouillet, Hélène Fricker-Hidalgo, Tiffany Lacharme, Jean-Paul Romanet, Christian Massot, Christophe Chiquet, Hervé Pelloux |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 51
Issue 11
Pg. 5530-6
(Nov 2010)
ISSN: 1552-5783 [Electronic] United States |
PMID | 20554617
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Protozoan
- HSP70 Heat-Shock Proteins
- Immunoglobulin G
- Immunoglobulin M
- heat-shock protein 70.1
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Animals
- Antibodies, Protozoan
(blood)
- Aqueous Humor
(parasitology)
- Blood Donors
- Case-Control Studies
- Child
- Enzyme-Linked Immunosorbent Assay
- Female
- HSP70 Heat-Shock Proteins
(immunology)
- Humans
- Immunoglobulin G
(blood)
- Immunoglobulin M
(blood)
- Male
- Middle Aged
- Retrospective Studies
- Toxoplasma
(immunology)
- Toxoplasmosis, Ocular
(diagnosis, immunology)
- Uveitis, Posterior
(diagnosis, immunology)
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