Abstract | OBJECTIVE: METHODS: The lung cancer tissue samples from 18 patients were cultured and 4 lung cell lines (A549, NCI-H446, SK-MES-1, BEAS-2B) were also included in the study. Air samples in the headspace of culture flasks were analyzed for VOCs with solid-phase micro-extraction and gas chromatography-mass spectroscopy technique ( SPME-GC/MS). RESULT: Two kinds of VOCs 2-pentadecanone and nonadecane were detected in lung cancer cell lines A549, NCI-H446 and SK-MES-1. The concentration of 2-pentadecanone were (1.382 + or -0.171) X 10(-5)mg/L, (1.681 + or - 0.190) X 10(-4)mg/L and (2.835 + or - 0.401) X 10(-6)mg/L, respectively; the concentrations of nonadecane were (8.382 + or - 0.606 ) X 10(-6)mg/L, (1.845 + or - 0.130) X 10(-5)mg/L and (6.220 + or - 0.362) X 10(-6)mg/L), respectively. The eicosane was detected in A549 and NCI-H446 with the concentration of (8.313 + or - 1.130) X 10(-6)mg/L and (1.020 + or - 0.141) X 10(-5)mg/L), respectively. All the 3 VOCs were not detected in cell line BEAS-2B. The concentrations of 12 VOCs including decane, 2- pentadecanone, nonadecane and eicosane were high in 18 lung cancer tissue samples; the concentrations of 2-pentadecanone were 5.421 X 10(-6)mg/L-3.621 X 10(-5)mg/L,those of nonadecane were 5.805 X 10(-6)mg/L-1.830 X 10(-5)mg/L, those of eicosane were 2.730 X 10(-6)mg/L-2.343 X 10(-5)mg/L. There were no differences of VOCs levels among patients with different cancer differentiation (P>0.05). The concentration of eicosane in the non- squamous carcinoma was higher than that in squamous carcinoma, the same results were confirmed in the lung cancer cell lines. CONCLUSION: This study has identified VOCs produced by lung cancer tissue, which may support to use breath test as a complementary noninvasive diagnostic method for lung cancer.
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Authors | Yan-jie Hu, Yuan-hua Qiu, En-guo Chen, Ke-jing Ying, Jin Yu, Ping Wang |
Journal | Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
(Zhejiang Da Xue Xue Bao Yi Xue Ban)
Vol. 39
Issue 3
Pg. 278-84
(05 2010)
ISSN: 1008-9292 [Print] China |
PMID | 20544990
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alkanes
- Biomarkers, Tumor
- Volatile Organic Compounds
- decane
- nonadecane
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Topics |
- Adult
- Aged
- Alkanes
(metabolism)
- Biomarkers, Tumor
(metabolism)
- Cell Line, Tumor
- Female
- Humans
- Lung Neoplasms
(diagnosis, metabolism)
- Male
- Middle Aged
- Tumor Cells, Cultured
- Volatile Organic Compounds
(metabolism)
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