Metabolomic analysis of thermally injured and/or septic rats.

Abstract

INTRODUCTION:

Early diagnosis and treatment for thermal injury with septic complications continue to be a serious clinical problem. In this study, plasma biomarkers of rats in the burn and/or septic models were investigated with a metabolomic method.

METHODS:

Rat plasma samples were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Multivariate analysis, the principal components analysis (PCA), was used to validate metabolic changes. In addition, another multivariate method, the orthogonal partial least-squares analysis (OPLS), was used to profile potential biomarkers in models.

RESULTS:

Nine characteristic metabolites, including hypoxanthine, indoxyl sufate, glucuronic acid, gluconic acid, proline, uracil, nitrotyrosine, uric acid, and trihydroxy cholanoic acid were identified in models of thermal injury and/or sepsis.

CONCLUSION:

These biomarkers were mainly involved in oxidative stress and tissue damage, and might supply evidence for distinguishing burned septic patients from non-septic ones.

• Authors: Xin-Ru Liu, Xing-Feng Zheng, Shi-Zhao Ji, Yong-Hai Lv, De-Yi Zheng, Zhao-Fan Xia, Wei-Dong Zhang
• Journal: Burns : journal of the International Society for Burn Injuries (Burns) Vol. 36 Issue 7 Pg. 992-8 (Nov 2010) ISSN: 1879-1409 [Electronic] Netherlands
• PMID: 20537801 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ltd and ISBI. All rights reserved.

Chemical References

• Amino Acids
• Biomarkers
• Carboxylic Acids
• Heterocyclic Compounds

Topics

• Amino Acids (blood)
• Animals
• Biomarkers (blood)
• Burns (blood)
• Carboxylic Acids (blood)
• Chromatography, High Pressure Liquid (methods)
• Disease Models, Animal
• Heterocyclic Compounds (blood)
• Male
• Mass Spectrometry
• Metabolomics (methods)
• Rats
• Rats, Sprague-Dawley
• Sepsis (blood, diagnosis)