Development of transferrin functionalized poly(ethylene glycol)/poly(lactic acid) amphiphilic block copolymeric micelles as a potential delivery system targeting brain glioma.
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| Abstract |
The aim of present study is to conceive a biodegradable poly(ethylene glycol)-polylactide (PEG-PLA) copolymer nanoparticle which can be surface biofunctionalized with ligands via biotin-avidin interactions and used as a potential drug delivery carrier targeting to brain glioma in vivo. For this aim, a new method was employed to synthesize biotinylated PEG-PLA copolymers, i.e., esterification of PEG with biotinyl chloride followed by copolymerization of hetero-biotinylated PEG with lactide. PEG-PLA nanoparticles bearing biotin groups on surface were prepared by nanoprecipitation technique and the functional protein transferrin (Tf) were coupled to the nanoparticles by taking advantage of the strong biotin-avidin complex formation. The flow cytometer measurement demonstrated the targeting ability of the nanoparticles to tumor cells in vitro, and the fluorescence microscopy observation of brain sections from C6 glioma tumor-bearing rat model gave the intuitive proof that Tf functionalized PEG-PLA nanoparticles could penetrate into tumor in vivo.
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| Authors | Wei-hua Ren, Jiang Chang, Cheng-hu Yan, Xiao-min Qian, Li-xia Long, Bin He, Xu-bo Yuan, Chun-sheng Kang, Didier Betbeder, Jing Sheng, Pei-yu Pu |
| Journal | Journal of materials science. Materials in medicine (J Mater Sci Mater Med) Vol. 21 Issue 9 Pg. 2673-81 (Sep 2010) ISSN: 1573-4838 [Electronic] United States |
| PMID | 20535631 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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