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Replication of TMV-L and Lta1 RNAs and their recombinants in TMV-resistant Tm-1 tomato protoplasts.

Abstract
Tm-1 is a gene that provides resistance to tomato plants against tobacco mosaic virus (TMV) infection. In tomato cells carrying the Tm-1 gene, multiplication of TMV is inhibited. From previous analysis of resistance-breaking mutants, the involvement of the 130- and 180-kDa proteins, putative viral replicases, in the resistance conferred by the Tm-1 gene was suggested. When wild-type TMV RNA was co-inoculated with a resistance-breaking mutant RNA, replication of the wild-type TMV genomic RNA could not be rescued by the 130- and 180-kDa proteins of a resistance-breaking strain, Lta1. To investigate how the putative resistance factor interacts with the 130- and 180-kDa proteins, we expressed the wild-type TMV protein sequence that is associated with the resistance-breaking phenomenon as part of a recombinant virus derived from Lta1 in Tm-1/Tm-1 protoplasts. No specific degradation of wild-type TMV protein sequences was observed, suggesting that the mechanism of the resistance does not involve the instability of a viral protein.
AuthorsR Yamafuji, Y Watanabe, T Meshi, Y Okada
JournalVirology (Virology) Vol. 183 Issue 1 Pg. 99-105 (Jul 1991) ISSN: 0042-6822 [Print] United States
PMID2053299 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Capsid Proteins
  • H protein, Tobacco mosaic virus
  • RNA, Viral
  • Recombinant Fusion Proteins
  • Viral Proteins
Topics
  • Amino Acid Sequence
  • Base Sequence
  • Capsid Proteins
  • Genes, Plant
  • Molecular Sequence Data
  • Molecular Weight
  • Plant Diseases
  • Protoplasts (microbiology)
  • RNA, Viral (biosynthesis)
  • Recombinant Fusion Proteins (biosynthesis, genetics)
  • Recombination, Genetic
  • Tobacco Mosaic Virus (genetics)
  • Viral Proteins (biosynthesis, genetics)
  • Virus Replication (genetics)

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