Abstract | BACKGROUND: MATERIALS AND METHODS: Here, 110 colorectal carcinomas of different grades and stages, including lymph node and liver metastases were compared to adjacent normal mucosa by immunohistochemistry of P150. Western blot analysis of selected cases confirmed the expression levels determined by immunohistochemistry. Additionally, immuno-electron and laser scanning microscopy (LSM) was performed. RESULTS: All investigated carcinomas revealed high levels of p150 protein compared to normal adjacent mucosa. The staining intensity was slightly heterogeneous, and positivity was correlated to the tumor grade with statistically significant differences of p150 expression between normal and neoplastic mucosa (p<0.0001, Kruskal-Wallis test). Western blots confirmed higher expression levels of p150 in the tumor. Immunogold labelling and LSM investigation showed high expression levels of p150 on the rough endoplasmic reticulum and polyribosomes, indicating that p150 is translationally active in these tumors. CONCLUSION:
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Authors | J Haybaeck, T O'Connor, R Spilka, G Spizzo, Ch Ensinger, G Mikuz, T Brunhuber, A Vogetseder, I Theurl, W Salvenmoser, H Draxl, R Bänziger, F Bachmann, G Schäfer, M Burger, P Obrist |
Journal | Anticancer research
(Anticancer Res)
Vol. 30
Issue 4
Pg. 1047-55
(Apr 2010)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 20530408
(Publication Type: Journal Article)
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Chemical References |
- EIF3A protein, human
- Eukaryotic Initiation Factor-3
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Topics |
- Cell Differentiation
(physiology)
- Colorectal Neoplasms
(metabolism, pathology)
- Eukaryotic Initiation Factor-3
(biosynthesis)
- Humans
- Immunoblotting
- Immunohistochemistry
- Microscopy, Confocal
- Microscopy, Electron
- Middle Aged
- Neoplasm Staging
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