Secretion of the chlamydial virulence factor CPAF requires the Sec-dependent pathway.

Abstract

The chlamydial protease/proteasome-like activity factor (CPAF) is secreted into the host cytosol to degrade various host factors that benefit chlamydial intracellular survival. Although the full-length CPAF is predicted to contain a putative signal peptide at its N terminus, the secretion pathway of CPAF is still unknown. Here, we have provided experimental evidence that the N-terminal sequence covering the M1-G31 region was cleaved from CPAF during chlamydial infection. The CPAF N-terminal sequence, when expressed in a phoA gene fusion construct, was able to direct the export of the mature PhoA protein across the inner membrane of wild-type Escherichia coli. However, E. coli mutants deficient in SecB failed to support the CPAF signal-peptide-directed secretion of PhoA. Since native PhoA secretion was known to be independent of SecB, this SecB dependence must be rendered by the CPAF leader peptide. Furthermore, lack of SecY function also blocked the CPAF signal-peptide-directed secretion of PhoA. Most importantly, CPAF secretion into the host cell cytosol during chlamydial infection was selectively inhibited by an inhibitor specifically targeting type I signal peptidase but not by a type III secretion-system-specific inhibitor. Together, these observations have demonstrated that the chlamydial virulence factor CPAF relies on Sec-dependent transport for crossing the chlamydial inner membrane, which has provided essential information for further delineating the pathways of CPAF action and understanding chlamydial pathogenic mechanisms.

• Authors: Ding Chen, Lei Lei, Chunxue Lu, Rhonda Flores, Matthew P DeLisa, Tucker C Roberts, Floyd E Romesberg, Guangming Zhong
• Journal: Microbiology (Reading, England) (Microbiology (Reading)) Vol. 156 Issue Pt 10 Pg. 3031-3040 (Oct 2010) ISSN: 1465-2080 [Electronic] England
• PMID: 20522495 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Bacterial Proteins
• Escherichia coli Proteins
• Protein Sorting Signals
• SecB protein, Bacteria
• Virulence Factors
• Alkaline Phosphatase
• phoA protein, E coli

Topics

• Alkaline Phosphatase (metabolism)
• Amino Acid Sequence
• Bacterial Proteins (genetics, metabolism)
• Chlamydia trachomatis (genetics, metabolism)
• Escherichia coli (genetics, metabolism)
• Escherichia coli Proteins (metabolism)
• HeLa Cells
• Humans
• Molecular Sequence Data
• Protein Sorting Signals
• Protein Transport
• Virulence Factors (genetics, metabolism)