Photosensitization of intact heart mitochondria by the phthalocyanine Pc 4: Correlation of structural and functional deficits with cytochrome c release.
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| Abstract |
Singlet oxygen is produced by the absorption of red light by the phthalocyanine dye Pc 4, followed by energy transfer to dissolved triplet oxygen. Mitochondria preincubated with Pc 4 were illuminated by red light and the damage to mitochondrial structure and function by the generated singlet oxygen was studied. At early illumination times (3-5 min of red light exposure), State 3 respiration was inhibited (50%), whereas State 4 activity increased, resulting in effectively complete uncoupling. Individual complex activities were measured and only complex IV activity was significantly reduced and exhibited a dose response, whereas the activities of electron transport complexes I, II, and III were not significantly affected. Cytochrome c release was an increasing function of irradiation time, with 30% being released after 5 min of illumination. Mitochondrial expansion along with changes in the structure of the cristae were observed by transmission electron microscopy after 5 min of irradiation, with an increase in large vacuoles and membrane rupture occurring after more extensive exposures.
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| Authors | Junhwan Kim, Hisashi Fujioka, Nancy L Oleinick, Vernon E Anderson |
| Journal | Free radical biology & medicine (Free Radic Biol Med) Vol. 49 Issue 5 Pg. 726-32 (Sep 01 2010) ISSN: 1873-4596 [Electronic] United States |
| PMID | 20510354 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural) |
| Copyright | Copyright 2010 Elsevier Inc. All rights reserved. |
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