Development of small molecules targeting the Wnt pathway for the treatment of colon cancer: a high-throughput screening approach.

Abstract

Wnt proteins play major roles in development and differentiation, and abnormalities in their regulation are believed to contribute to the formation of many cancers, including colorectal malignancies. As a result, there has been an interest in identifying small molecule inhibitors of Wnt signaling as tool compounds for research or as precursors to new generations of anticancer drugs. Advancements in robotic technology along with reductions in the costs of equipment, chemical libraries, and information handling have made high-throughput drug discovery programs possible in an academic setting. In this minireview we discuss the most plausible protein targets for inhibiting Wnt signaling in colon cancer therapy, list small molecule Wnt inhibitors that have been identified through recent drug discovery efforts, and provide our laboratory's strategy for identifying novel Wnt signaling antagonists using high-throughput screening. In particular, we summarize the results of a screen of over 1,200 drug and druglike compounds we recently completed in which niclosamide was identified as a Wnt pathway antagonist.

• Authors: Wei Chen, Minyong Chen, Larry S Barak
• Journal: American journal of physiology. Gastrointestinal and liver physiology (Am J Physiol Gastrointest Liver Physiol) Vol. 299 Issue 2 Pg. G293-300 (Aug 2010) ISSN: 1522-1547 [Electronic] United States
• PMID: 20508156 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review)

Chemical References

• Anthelmintics
• Wnt Proteins
• Niclosamide

Topics

• Anthelmintics (pharmacology)
• Colonic Neoplasms (drug therapy)
• High-Throughput Screening Assays
• Humans
• Niclosamide (pharmacology)
• Signal Transduction (drug effects)
• Wnt Proteins (antagonists & inhibitors, metabolism)