[Inhibitory effect of SHP-1 gene transfer on the proliferation of breast cancer cell line MDA-MB-231].

Abstract

OBJECTIVE:

To observe SHP-1 protein expression in breast cancer cell line MDA-MB-231 before and after SHP-1 gene transfer and its effect on the proliferation of MDA-MB-231 cells.

METHODS:

The eukaryotic expression vector pEGFP-C3-SHP-1 was constructed and transfected into breast cancer cell line MDA-MB-231 via Lipofectamine 2000, and the positive clones were selected using G418. SHP-1 expression in MDA-MB-231 cells was detected with immunocytochemistry and Western blotting, and the cell growth curve was observed using MTT assay.

RESULTS:

SHP-1 was highly expressed in transfected MDA-MB-231 cells, whose proliferation was significantly inhibited (P<0.05).

CONCLUSION:

SHP-1 gene transfer into MDA-MB-231 cells results in inhibition of the cell proliferation.

• Authors: Ya-lin Xie, Ji-zhen Liang, Fei-ye Liu, Jun-yi Zhang, Jian-ming Xie, Rong-cheng Luo, Rong Li
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 30 Issue 5 Pg. 1024-7 (May 2010) ISSN: 1673-4254 [Print] China
• PMID: 20501384 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• PTPN6 protein, human
• Protein Tyrosine Phosphatase, Non-Receptor Type 6

Topics

• Breast Neoplasms (genetics, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Female
• Gene Transfer Techniques
• Humans
• Protein Tyrosine Phosphatase, Non-Receptor Type 6 (genetics, metabolism)