Ovalbumin (OVA) is widely used in
allergy research. OVA
peptide 323-339 has been reported to be responsible for 25-35% of isolated BALB/c mouse T-cell response to intact OVA. An investigation of whether OVA and
OVA 323-339 molecules can induce equivalent in vivo and in vitro immune responses was conducted. Eight-week-old BALB/c mice were randomly divided into three groups: OVA,
OVA 323-339 and saline. On days 0, 7, 14, mice were intraperitoneally injected with 25 microg OVA or
OVA 323-339 absorbed on 300 microg
Alum, or saline; on days 21-23, all groups were challenged intranasally with either 20 microl of 1% OVA, 1%
OVA 323-339 or saline. On day 28, after killing, splenocytes were isolated and cultured under the stimulus of each
allergen or medium. Evaluated by
hematoxylin/
eosin and major basic
protein immunohistochemical stainings, OVA and
OVA 323-339 induced similar
lung inflammation. Interestingly, significant serum total
IgE and OVA-specific
IgE were observed in OVA mice when compared to saline control.
OVA 323-339 mice showed higher serum OVA-specific
IgE, OVA 323-339-specific
IgE,
IL-4 and lower IFN-gamma similar to OVA mice. The proliferative response to OVA was found in cultured splenocytes of both OVA and
OVA 323-339 mice, while the similar proliferative response to
OVA 323-339 was only observed in the splenocytes of OVA 323-339-sensitized and challenged mice. Although
OVA 323-339 induced a Th2-like response in the mouse model as did OVA,
OVA 323-339 has clearly limited immunogenic potency to activate OVA-sensitized and challenged mice splenocytes, unlike OVA.