Anti-inflammatory effects of limonene from yuzu (Citrus junos Tanaka) essential oil on eosinophils.

Abstract

Yuzu (Citrus junos Tanaka) has been used as a traditional medicine in Japan. We investigated in vitro anti-inflammatory effects of limonene from yuzu peel on human eosinophilic leukemia HL-60 clone 15 cells. To examine anti-inflammatory effects of limonene on the cells, we measured the level of reactive oxygen species (ROS), monocyte chemoattractant protein-1 (MCP-1), nuclear factor (NF) kappa B, and p38 mitogen-activated protein kinase (MAPK). We found that low concentration of limonene (7.34 mmol/L) inhibited the production of ROS for eotaxin-stimulated HL-60 clone 15 cells. 14.68 mmol/L concentration of limonene diminished MCP-1 production via NF-kappa B activation comparable to the addition of the proteasomal inhibitor MG132. In addition, it inhibited cell chemotaxis in a p38 MAPK dependent manner similar to the adding of SB203580. These results suggest that limonene may have potential anti-inflammatory efficacy for the treatment of bronchial asthma by inhibiting cytokines, ROS production, and inactivating eosinophil migration.

• Authors: Ryoji Hirota, Ngatu Nlandu Roger, Hiroyuki Nakamura, Hee-Sun Song, Masayoshi Sawamura, Narufumi Suganuma
• Journal: Journal of food science (J Food Sci) Vol. 75 Issue 3 Pg. H87-92 (Apr 2010) ISSN: 1750-3841 [Electronic] United States
• PMID: 20492298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• CCL2 protein, human
• Chemokine CCL2
• Cyclohexenes
• DNA-Binding Proteins
• NF-kappa B
• Oils, Volatile
• Reactive Oxygen Species
• Terpenes
• Limonene
• p38 Mitogen-Activated Protein Kinases

Topics

• Anti-Inflammatory Agents (pharmacology)
• Asthma (drug therapy)
• Cell Movement (drug effects)
• Chemokine CCL2 (metabolism)
• Citrus (chemistry)
• Cyclohexenes (pharmacology)
• DNA-Binding Proteins (metabolism)
• Dose-Response Relationship, Drug
• Eosinophils (drug effects, immunology, metabolism)
• Fruit (chemistry)
• HL-60 Cells
• Humans
• Japan
• Limonene
• Medicine, East Asian Traditional
• NF-kappa B (metabolism)
• Oils, Volatile (chemistry)
• Phosphorylation (drug effects)
• Reactive Oxygen Species (metabolism)
• Terpenes (pharmacology)
• p38 Mitogen-Activated Protein Kinases (metabolism)