Chemopreventive properties of phytosterols and maslinic acid extracted from Coleus tuberosus in inhibiting the expression of EBV early-antigen in Raji cells.

Abstract

Bioassay-guided fractionation of a MeOH extract of tubers of Coleus tuberosus afforded the active anti-tumor-promoting compounds identified as the triterpenoid 2alpha,3beta-dihydroxyolean-12-en-28-oic acid (maslinic acid; CT2) and a phytosterol mixture (CT1). CT1 consists of stigmasterol (32%), beta-sitosterol (40.3%), and campesterol (27.7%) as determined by capillary gas chromatography. CT1 and CT2 showed very strong anti-tumor-promoting activities at IC(50) 0.7 microg/ml and 0.1 microg/ml, respectively, in a convenient, short-term in vitro assay, i.e., the inhibition of Epstein-Barr virus (EBV) activation induced by phorbol 12-myristate 13-acetate (PMA) and sodium butyrate. We report for the first time the anti-tumor-promoting activity of 2alpha,3beta-dihydroxyolean-12-en-28-oic acid and show that a mixture of stigmasterol, beta-sitosterol, and campesterol is more potent than the individual components in inhibiting tumor-promoting activity.

• Authors: Lim Yang Mooi, Norhanom Abdul Wahab, Nordin Haji Lajis, Abdul Manaf Ali
• Journal: Chemistry & biodiversity (Chem Biodivers) Vol. 7 Issue 5 Pg. 1267-75 (May 2010) ISSN: 1612-1880 [Electronic] Switzerland
• PMID: 20491082 (Publication Type: Journal Article)

Chemical References

• Anticarcinogenic Agents
• Antigens, Viral
• Antineoplastic Agents, Phytogenic
• Butyrates
• Epstein-Barr virus early antigen
• Phytosterols
• Triterpenes
• 4-O-methyl-12-O-tetradecanoylphorbol 13-acetate
• maslinic acid
• Tetradecanoylphorbol Acetate

Topics

• Anticarcinogenic Agents (chemistry, isolation & purification, pharmacology)
• Antigens, Viral (metabolism)
• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
• Butyrates (pharmacology)
• Cell Line
• Coleus (chemistry)
• Herpesvirus 4, Human (drug effects)
• Humans
• Molecular Conformation
• Phytosterols (chemistry, isolation & purification, pharmacology)
• Plant Tubers (chemistry)
• Tetradecanoylphorbol Acetate (analogs & derivatives, pharmacology)
• Triterpenes (chemistry, isolation & purification, pharmacology)
• Virus Activation (drug effects)