Nogo-A expression in oligodendroglial tumors.

Abstract

Nogo-A belongs to the reticulon protein family and is expressed in the inner and outer loops of myelin sheaths of oligodendrocytes. We analyzed the patterns of Nogo-A expression in human gliomas in an effort to identify a useful marker for the characterization of oligodendroglial tumors. We determined the expression of Nogo-A in a panel of 58 astrocytic and oligodendroglial tumors using immunohistochemistry and compared the expression of Nogo-A with Olig-2, a recently identified marker for oligodendrogliomas. To localize Nogo-A expression, immunofluorescent staining was performed using other glial markers (MAP-2 and GFAP). We also confirmed the overexpression of the Nogo-A protein in 53 astrocytic and oligodendroglial tumors using Western blot analysis. Based on immunohistochemical analysis, Nogo-A and Olig-2 had specificity in the detection of oligodendroglial tumors from astrocytic tumors (P=0.001). The level of Nogo-A staining was highly correlated with Olig-2 (P=0.001). The sensitivity and specificity of Nogo-A for oligodendroglial tumors was 86.9% and 57.1%, respectively. Nogo-A expression overlapped that of other oligodendroglial markers, but with different patterns of expression. Western blot analysis revealed that Nogo-A is predominantly expressed in 85.7% of oligodendroglioma cells and 93.7% of anaplastic oligodendroglioma cells. Like other oligodendroglial markers, Nogo-A is highly expressed in oligodendroglial tumors; however, it does not serve as a definite marker specific for oligodendroglial tumors.

• Authors: Tae-Young Jung, Shin Jung, Kyung-Hwa Lee, Van Thang Cao, Shu-Guang Jin, Kyung-Sub Moon, In-Young Kim, Sam-Suk Kang, Hyung-Seok Kim, Min-Cheol Lee
• Journal: Neuropathology : official journal of the Japanese Society of Neuropathology (Neuropathology) Vol. 31 Issue 1 Pg. 11-9 (Feb 2011) ISSN: 1440-1789 [Electronic] Australia
• PMID: 20487307 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2010 Japanese Society of Neuropathology.

Chemical References

• Biomarkers, Tumor
• Myelin Proteins
• Nogo Proteins
• RTN4 protein, human

Topics

• Astrocytoma (metabolism, pathology)
• Biomarkers, Tumor (analysis)
• Blotting, Western
• Brain Neoplasms (metabolism, pathology)
• Glioma (metabolism, pathology)
• Humans
• Immunohistochemistry
• In Situ Hybridization, Fluorescence
• Microscopy, Fluorescence
• Myelin Proteins (biosynthesis)
• Nogo Proteins
• Oligodendroglioma (metabolism, pathology)
• Sensitivity and Specificity