Abstract | INTRODUCTION: MATERIALS AND METHODS: A fusion gene encoding r-RGD-hirudin was constructed and expressed at high levels in Pichia pastoris. Following traumatic injury and anastomosis, 42 New Zealand White rabbits were randomized to receive normal saline, abciximab, wild-type hirudin, or r-RGD-hirudin. Fibrinogen concentration, aPTT, TT, PT, and PAGm were measured prior to and following the operation. Carotid angiography and pathological examination of the anastomotic site were performed to compare patency rates among the groups. RESULTS: The r-RGD-hirudin significantly prolonged aPTT, TT, PT and inhibited PAGm following carotid anastomosis in rabbits. The median dose of r-RGD-hirudin (0.5 mg/kg) had a therapeutic effect equal to that of wild-type hirudin (1.0 mg/kg) and higher than that of abciximab (0.2 mg/kg) with regard to patency rates. CONCLUSIONS:
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Authors | Wei Feng Lu, Wei Mo, Zhenjie Liu, Wei Guo Fu, Da Qiao Guo, Yu Qi Wang, Hou Yan Song |
Journal | Thrombosis research
(Thromb Res)
Vol. 126
Issue 4
Pg. e339-43
(Oct 2010)
ISSN: 1879-2472 [Electronic] United States |
PMID | 20483158
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Ltd. All rights reserved. |
Chemical References |
- Anticoagulants
- Antithrombins
- Hirudins
- Platelet Aggregation Inhibitors
- RGD-hirudin
- Recombinant Proteins
- Thrombin
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Topics |
- Animals
- Anticoagulants
(pharmacology, therapeutic use)
- Antithrombins
(pharmacology, therapeutic use)
- Carotid Arteries
(drug effects, pathology)
- Gene Expression
- Hirudins
(genetics, pharmacology)
- Male
- Pichia
(genetics)
- Platelet Aggregation
(drug effects)
- Platelet Aggregation Inhibitors
(pharmacology, therapeutic use)
- Rabbits
- Recombinant Proteins
(pharmacology, therapeutic use)
- Thrombin
(antagonists & inhibitors, metabolism)
- Thrombosis
(drug therapy)
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