Platelet P-selectin reflects a state of cutaneous inflammation: possible application to monitor treatment efficacy in psoriasis.

Abstract

Haemostasis-maintaining platelets are also recognized as important modulators in the regulation of immune response. Activated platelets expressing P-selectin (CD62P) are involved in the extravasation of leucocytes. This study evaluated platelet P-selectin expression as a biomarker for cutaneous inflammation. P-selectin expression was assessed by flow cytometry in 147 successive patients suffering from an inflammatory or infectious skin condition at the day of admission for in-patient treatment as well as a day prior to demission. Forty-one patients admitted for allergy testing served as controls. A commercially available ELISA was used in 17 patients to determine soluble P-selectin in the plasma. In patients with psoriasis, the Psoriasis Area and Severity Index (PASI) was documented as a measure for disease severity. We observed a significant increase in platelet P-selectin expression in patients with inflammatory or infectious disorders, when compared to the control group (3,01% vs. 1,46%; P < 0.000001). Successful treatment resulted in a significant decrease in P-selectin expression to the level of the control group. In the case of psoriasis (n = 47), we found highly significant correlation between P-selectin and PASI (r = 0.51; P < 0.000001), as well as between the change in the PASI and the change in P-selectin expression (r = 0.4; P = 0.006). Platelet P-selectin expression as determined by flow cytometry correlated well with the results of soluble P-selectin, determined by ELISA (r = 0.63; P < 0.01). Thus, platelet P-selectin expression may be used as an efficacy biomarker to monitor treatment success in psoriasis. As platelet P-selectin correlates with soluble P-selectin in patient plasma, which can be measured by ELISA, the latter is feasible also for routine use.

• Authors: Jurate Garbaraviciene, Sandra Diehl, Domenica Varwig, Matilda Bylaite, Hanns Ackermann, Ralf J Ludwig, Wolf-Henning Boehncke
• Journal: Experimental dermatology (Exp Dermatol) Vol. 19 Issue 8 Pg. 736-41 (Aug 2010) ISSN: 1600-0625 [Electronic] Denmark
• PMID: 20482619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Biomarkers
• P-Selectin
• C-Reactive Protein

Topics

• Anti-Inflammatory Agents (therapeutic use)
• Biomarkers (blood)
• Blood Platelets (metabolism)
• C-Reactive Protein (metabolism)
• Case-Control Studies
• Dermatitis (blood)
• Humans
• P-Selectin (blood)
• Phototherapy
• Psoriasis (blood, diagnosis, therapy)
• Severity of Illness Index
• Treatment Outcome